• Absorbance analysis at 450nm using AR ELISA Kit.

AR Competitive ELISA Kit (STJ150094)

SKU:
STJ150094

Current Stock:
Applications: ELISA
Reactivity: Human
Short Description: This AR Competitive ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Note: FOR RESEARCH USE ONLY (RUO).
Sensitivity: 20ng/mL
Detection Limit: 0.313--20ng/mL
Storage Instruction: Store at 4°C for 6 months.
Uniprot ID: ANDR_HUMAN
Gene ID: 367
Gene Symbol: AR
Sample Type: Serum, Plasma, Biological Fluids
Post Translational Modifications Sumoylated on Lys-388 (major) and Lys-521. Ubiquitinated. Deubiquitinated by USP26. 'Lys-6' and 'Lys-27'-linked polyubiquitination by RNF6 modulates AR transcriptional activity and specificity. Phosphorylated in prostate cancer cells in response to several growth factors including EGF. Phosphorylation is induced by c-Src kinase (CSK). Tyr-535 is one of the major phosphorylation sites and an increase in phosphorylation and Src kinase activity is associated with prostate cancer progression. Phosphorylation by TNK2 enhances the DNA-binding and transcriptional activity and may be responsible for androgen-independent progression of prostate cancer. Phosphorylation at Ser-83 by CDK9 regulates AR promoter selectivity and cell growth. Phosphorylation by PAK6 leads to AR-mediated transcription inhibition. Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
Function Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins like ZBTB7A that recruits NCOR1 and NCOR2 to the androgen response elements/ARE on target genes, negatively regulating androgen receptor signaling and androgen-induced cell proliferation. Transcription activation is also down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3. Isoform 3 and isoform 4 lack the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.
Protein Name Androgen Receptor
Dihydrotestosterone Receptor
Nuclear Receptor Subfamily 3 Group C Member 4
Database Links Reactome: R-HSA-3371497
Reactome: R-HSA-383280
Reactome: R-HSA-4090294
Reactome: R-HSA-5625886
Reactome: R-HSA-5689880
Reactome: R-HSA-8940973
Cellular Localisation Nucleus
Cytoplasm
Detected At The Promoter Of Target Genes
Predominantly Cytoplasmic In Unligated Form But Translocates To The Nucleus Upon Ligand-Binding
Can Also Translocate To The Nucleus In Unligated Form In The Presence Of Rack1
Alternative ELISA Kit Names Androgen Receptor ELISA kit
Dihydrotestosterone Receptor ELISA kit
Nuclear Receptor Subfamily 3 Group C Member 4 ELISA kit
AR ELISA kit
DHTR ELISA kit
NR3C4 ELISA kit

Information sourced from Uniprot.org

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