• Immunofluorescence analysis of A-549 cells using ZNF148 antibody (STJ29081).
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using ZNF148 antibody (STJ29081) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using ZNF148 antibody (STJ29081) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using ZNF148 antibody (STJ29081) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using ZNF148 Antibody (STJ29081) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ZNF148 antibody (690-794) (STJ29081)

SKU:
STJ29081

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ZNF148 (690-794) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
IF/ICC 1:20-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ZNF148
Gene ID: 7707
Uniprot ID: ZN148_HUMAN
Immunogen Region: 690-794
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 690-794 of human ZNF148 (NP_068799.2).
Immunogen Sequence: SGDETNHASATSTQDFLDQV TSQKKAEAQPVHQAYQMSSF EQPFRAPYHGSRAGIATQFS TANGQVNLRGPGTSAEFSEF PLVNVNDNRAGMTSSPDATT GQTFG
Post Translational Modifications Sumoylated with SUMO2. Desumoylated by SENP3, resulting in the stimulation of transcription of its target genes.
Function Involved in transcriptional regulation. Represses the transcription of a number of genes including gastrin, stromelysin and enolase. Binds to the G-rich box in the enhancer region of these genes.
Protein Name Zinc Finger Protein 148
Transcription Factor Zbp-89
Zinc Finger Dna-Binding Protein 89
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Zinc Finger Protein 148 antibody
Anti-Transcription Factor Zbp-89 antibody
Anti-Zinc Finger Dna-Binding Protein 89 antibody
Anti-ZNF148 antibody
Anti-ZBP89 antibody

Information sourced from Uniprot.org

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