• Western blot analysis of extracts of various cell lines, using 14-3-3 epsilon rabbit monoclonal antibody (STJ11102200) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-YWHAE antibody (14-3) [ARC1222] (STJ11102200)

SKU:
STJ11102200

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Host: Rabbit
Applications: WB
Reactivity: Human, Mouse, Rat
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit monoclonal antibody anti-14-3-3 epsilon (14-3) is suitable for use in Western Blot.
Clonality: Monoclonal
Clone ID: ARC1222
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS containing 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store in a freezer at-20°C and avoid freeze-thaw cycles.
Gene Symbol: YWHAE
Gene ID: 7531
Uniprot ID: 1433E_HUMAN
Immunogen Region: 14-3
Immunogen: A synthesized peptide derived from human 14-3-3 epsilon
Function Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm.
Protein Name 14-3-3 Protein Epsilon
14-3-3e
Database Links Reactome: R-HSA-111447
Reactome: R-HSA-1445148
Reactome: R-HSA-2028269
Reactome: R-HSA-205025
Reactome: R-HSA-2565942
Reactome: R-HSA-3371453
Reactome: R-HSA-3371511
Reactome: R-HSA-380259
Reactome: R-HSA-380270
Reactome: R-HSA-380284
Reactome: R-HSA-380320
Reactome: R-HSA-5620912
Reactome: R-HSA-5625740
Reactome: R-HSA-5628897
Reactome: R-HSA-75035
Reactome: R-HSA-8854518
Reactome: R-HSA-8862803
Reactome: R-HSA-8876198
Cellular Localisation Nucleus
Cytoplasm
Melanosome
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-14-3-3 Protein Epsilon antibody
Anti-14-3-3e antibody
Anti-YWHAE antibody

Information sourced from Uniprot.org

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