• Western blot analysis of extracts of various cell lines, using 14-3-3 alpha/beta rabbit monoclonal antibody (STJ11102440) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-YWHAB antibody [ARC1453] (STJ11102440)


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Host: Rabbit
Applications: WB
Reactivity: Human, Mouse, Rat
Short Description: Rabbit monoclonal antibody anti-14-3-3 alpha/beta is suitable for use in Western Blot.
Clonality: Monoclonal
Clone ID: ARC1453
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS containing 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store in a freezer at-20°C and avoid freeze-thaw cycles.
Gene Symbol: YWHAB
Gene ID: 7529
Uniprot ID: 1433B_HUMAN
Immunogen: A synthesized peptide derived from human 14-3-3 alpha/beta
Post Translational Modifications The alpha, brain-specific form differs from the beta form in being phosphorylated. Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.
Function Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13.
Protein Name 14-3-3 Protein Beta/Alpha
Protein 1054
Protein Kinase C Inhibitor Protein 1
Kcip-1 Cleaved Into - 14-3-3 Protein Beta/Alpha - N-Terminally Processed
Database Links Reactome: R-HSA-111447
Reactome: R-HSA-1445148
Reactome: R-HSA-165159
Reactome: R-HSA-166208
Reactome: R-HSA-170968
Reactome: R-HSA-170984
Reactome: R-HSA-2028269
Reactome: R-HSA-392517
Reactome: R-HSA-450385
Reactome: R-HSA-450513
Reactome: R-HSA-5625740
Reactome: R-HSA-5628897
Reactome: R-HSA-5673000
Reactome: R-HSA-5674135
Reactome: R-HSA-5675221
Reactome: R-HSA-6802946
Reactome: R-HSA-6802948
Reactome: R-HSA-6802952
Reactome: R-HSA-6802955
Reactome: R-HSA-75035
Reactome: R-HSA-9614399
Reactome: R-HSA-9649948
Reactome: R-HSA-9656223
Reactome: R-HSA-9660537
Cellular Localisation Cytoplasm
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Vacuole Membrane
(Microbial Infection) Upon Infection With Chlamydia Trachomatis
This Protein Is Associated With The Pathogen-Containing Vacuole Membrane Where It Colocalizes With Incg
Alternative Antibody Names Anti-14-3-3 Protein Beta/Alpha antibody
Anti-Protein 1054 antibody
Anti-Protein Kinase C Inhibitor Protein 1 antibody
Anti-Kcip-1 Cleaved Into - 14-3-3 Protein Beta/Alpha - N-Terminally Processed antibody
Anti-YWHAB antibody

Information sourced from Uniprot.org

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