• Western blot analysis of extracts of various cell lines, using WNT5B antibody (STJ110611) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using WNT5B antibody (STJ110611) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat lung using WNT5B antibody (STJ110611) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using WNT5B antibody (STJ110611) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat bone marrow using WNT5B antibody (STJ110611) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded rat bone marrow using WNT5B antibody (STJ110611) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat heart using WNT5B antibody (STJ110611) at dilution of 1:100 (40x lens).
  • Western blot analysis of Mouse ovary, using WNT5B antibody (STJ110611) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.
  • Immunohistochemistry of paraffin-embedded human lung cancer using WNT5B antibody (STJ110611) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human liver cancer using WNT5B antibody (STJ110611) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human colon carcinoma using WNT5B antibody (STJ110611) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human stomach using WNT5B antibody (STJ110611) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse testis leydig using WNT5B antibody (STJ110611) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse spleen using WNT5B antibody (STJ110611) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse kidney using WNT5B antibody (STJ110611) at dilution of 1:100 (40x lens).

Anti-WNT5B antibody (181-342) (STJ110611)

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STJ110611

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-WNT5B (181-342) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WNT5B
Gene ID: 81029
Uniprot ID: WNT5B_HUMAN
Immunogen Region: 181-342
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 181-342 of human WNT5B (NP_110402.2).
Immunogen Sequence: NLQNNEAGRRAVYKMA
Post Translational Modifications Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.
Function Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
Protein Name Protein Wnt-5b
Database Links Reactome: R-HSA-3238698
Reactome: R-HSA-4086400
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Protein Wnt-5b antibody
Anti-WNT5B antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance