• Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4ug/ml). IgG control: Unimmunized goat IgG (black line) followed
  • STJ73113 (0.1µg/ml) staining of Mouse (A) and (2µg/ml) Rat (B) Ovary lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • STJ73113 (2µg/ml) staining of HeLa (A) and Jurkat (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • STJ73113 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/Intermediate filament staining. The nuc
  • Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4ug/ml). IgG control: Unimmunized goat IgG (black line) followed
  • STJ73113 (0.1µg/ml) staining of Mouse (A) and (2µg/ml) Rat (B) Ovary lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • STJ73113 (2µg/ml) staining of HeLa (A) and Jurkat (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • STJ73113 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/Intermediate filament staining. The nuc

Anti-Vimentin Antibody (STJ73113)

SKU:
STJ73113

Current Stock:
Host: Goat
Applications: Pep-ELISA, WB, FC, IF, IHC
Reactivity: Human, Mouse, Rat, Dog, Pig, Bovine
Short Description: Goat polyclonal anti-Vimentin antibody is suitable for use in ELISA, Western Blot, Flow Cytometry, Immunofluorescence and Immunohistochemistry.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at-20°C. Minimize freezing and thawing.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage Instruction: Store at-20°C and avoid freeze-thaw cycles.
Concentration: 0.5mg/mL
Isotype: IgG
Dilution Range: WB-0.1-2µg/ml
IHC-5µg/ml
IF-Strong expression of the protein seen in the cytoplasm/Intermediate filaments of U2OS cells. 5µg/ml
FC-Flow cytometric analysis of HeLa cells. 10ug/ml
ELISA-antibody detection limit dilution 1:4000.
Uniprot ID: VIME_MOUSE
VIME_RAT
Gene ID: 22352
81818
Gene Symbol: Vim
Vim
Immunogen Region: C Terminus
Immunogen Sequence: QVINETSQHHDDLE
Accession Number: NP_003371.2
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance