• Anti-V5 antibody at 1:1000 dilution; MBP-V5 recombinant protein and 293 cells transfected with V5-mCherry; lysate at 100 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution
  • Myo5a deficient melanocytes transiently transduced with adenovirus expressing V5-tagged Myo5a, fixed and stained with anti-V5 antibody (1:1000) ; donkey anti-goat Alexa 568 labelled secondary antibody was used
  • Immunofluorescence – anti-V5 antibody using hCEC cells transduced with V5-mCherry-Rab11a; cells were fixed with methanol and anti-V5 at 1/100

Anti-Paramyxovirus sv5 (P&V) antibody (STJ140012)

SKU:
STJ140012

£85.50 - £532.50
Current Stock:
Host: Goat
Applications: WB/IF
Reactivity: V5
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-P and V proteins of the Paramyxovirus of simian virus SV5 is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS, 20% Glycerol and 0.05% Sodium Azide.
Purification: This antibody is epitope-affinity purified from goat antiserum.
Concentration: 3 mg/ml
Dilution Range: WB 1:500-1:5000
IF 1:50-1:2000
Catarino S Ribeiro-Rodrigues TM Sa Ferreira R et al. Cells 2020 Apr. PMID: 32272685
Calado J Santos AR Aires I et al. FEBS Lett 2018 Aug. PMID: 30156268
Storage Instruction: For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Specificity: Reacts specifically with V5-tagged recombinant fusion proteins expressed in transfected mammalian cells or produced by in vitro translation.
Immunogen: Purified recombinant peptide produced in E. coli.
Immunogen Sequence: MGKPIPNPLLGLDSTNPMGK PIPNPLLGLDSTNPMGKPIP NPLLGLDSTNPMGKPIPNPL LGLDSTNPMGKPIPNPLLGL DSTNPGRLERPHRD
Background Goat polyclonal antibody to V5 epitope. V5 peptide corresponds to amino acid residues GKPIPNPLLGLDST (95-108 aa) of the P and V proteins of the Paramyxovirus of simian virus SV5.

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance