• WB on mouse brain lysate. Blocking with 1% LFDM for 30 min at RT; Primary antibody used at 1: 1000 dilution incubated overnight at 4C.
  • IHC-P on paraffin sections of rat spinal cord.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions; DAB chromogen. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.Small neurons are stained and also some nuclear staining is observed.
  • IHC-P on paraffin sections of rat spinal cord.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions; DAB chromogen. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.Small neurons are stained and also some nuclear staining is observed.
  • IHC-P on paraffin sections of rat spinal cord.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions; DAB chromogen. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.Small neurons are stained and also some nuclear staining is observed.
  • IHC-P on paraffin sections of rat spinal cord.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions; DAB chromogen. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.Small neurons are stained and also some nuclear staining is observed.
  • IHC-P on paraffin sections of rat spinal cord.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions; DAB chromogen. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.Small neurons are stained and also some nuclear staining is observed.
  • IHC-P on paraffin sections of rat spinal cord.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions; DAB chromogen. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.Small neurons are stained and also some nuclear staining is observed.
  • IHC-P on paraffin sections of rat DRG.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions; DAB chromogen. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.Small neurons are stained and also some nuclear staining is observed.
  • IHC-P on paraffin sections of rat DRG.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions; DAB chromogen. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.Small neurons are stained and also some nuclear staining is observed.
  • IHC-P on paraffin sections of rat DRG.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions; DAB chromogen. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.Small neurons are stained and also some nuclear staining is observed.
  • IHC-P on paraffin sections of rat DRG.The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions; DAB chromogen. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.Small neurons are stained and also some nuclear staining is observed.

Anti-UCHL1 antibody (50-100) (STJ13100235)

SKU:
STJ13100235

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Host: NZ White Rabbit
Applications: IHC, WB
Reactivity: Human, Mouse, Rat
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: NZ White Rabbit polyclonal antibody Anti-PGP 9.5 (50-100) is suitable for use in Immunohistochemistry and Western Blot.
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Shipped as lyophilised. Reconstitute in 150 µl of sterile water. Centrifuge to remove any insoluble material.
Purification: Whole serum
Dilution Range: A dilution of 1:1000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Storage Instruction: Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Gene Symbol: UCHL1
Gene ID: 7345
Uniprot ID: UCHL1_HUMAN
Immunogen Region: 50-100
Immunogen: A synthetic peptide from aa region 50-100 of mouse PGP 9.5 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and human.
Post Translational Modifications O-glycosylated.
Function Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins (Probable). This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
Protein Name Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1
Uch-L1
Neuron Cytoplasmic Protein 9.5
Pgp 9.5
Pgp9.5
Ubiquitin Thioesterase L1
Database Links Reactome: R-HSA-5689603
Cellular Localisation Cytoplasm
Endoplasmic Reticulum Membrane
Lipid-Anchor
About 30% Of Total Uchl1 Is Associated With Membranes In Brain
Alternative Antibody Names Anti-Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1 antibody
Anti-Uch-L1 antibody
Anti-Neuron Cytoplasmic Protein 9.5 antibody
Anti-Pgp 9.5 antibody
Anti-Pgp9.5 antibody
Anti-Ubiquitin Thioesterase L1 antibody
Anti-UCHL1 antibody

Information sourced from Uniprot.org

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