• Western blot analysis of lysates from PC12 cells, using TRPV4 antibody.
  • Western blot analysis of extracts from K562 cells, using TRPV4 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-TRPV4 antibody (417-466 aa) (STJ96405)

SKU:
STJ96405

Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Transient receptor potential cation channel subfamily V member 4 (417-466 aa) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TRPV4
Gene ID: 59341
Uniprot ID: TRPV4_HUMAN
Immunogen Region: 417-466 aa
Specificity: TRPV4 Polyclonal Antibody detects endogenous levels of TRPV4 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human TRPV4 at the amino acid range 417-466
Post Translational Modifications N-glycosylated.
Function Non-selective calcium permeant cation channel involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by heat, low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers. Acts as a regulator of intracellular Ca(2+) in synoviocytes. Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8. Together with PKD2, forms mechano- and thermosensitive channels in cilium. Negatively regulates expression of PPARGC1A, UCP1, oxidative metabolism and respiration in adipocytes. Regulates expression of chemokines and cytokines related to pro-inflammatory pathway in adipocytes. Together with AQP5, controls regulatory volume decrease in salivary epithelial cells. Required for normal development and maintenance of bone and cartilage. In its inactive state, may sequester DDX3X at the plasma membrane. When activated, the interaction between both proteins is affected and DDX3X relocalizes to the nucleus. Isoform 5: Non-selective calcium permeant cation channel involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by phorbol esters. Has the same channel activity as isoform 1, and is activated by the same stimuli. Isoform 2: Lacks channel activity, due to impaired oligomerization and intracellular retention. Isoform 4: Lacks channel activity, due to impaired oligomerization and intracellular retention. Isoform 6: Lacks channel activity, due to impaired oligomerization and intracellular retention. (Microbial infection) Facilitates hepatitis C virus (HCV) replication, possibly through its action on DDX3X. (Microbial infection) Facilitates Dengue virus (DENV) replication, possibly through its action on DDX3X. (Microbial infection) Facilitates Zika virus (ZIKV) replication, possibly through its action on DDX3X.
Protein Name Transient Receptor Potential Cation Channel Subfamily V Member 4
Trpv4
Osm-9-Like Trp Channel 4
Otrpc4
Transient Receptor Potential Protein 12
Trp12
Vanilloid Receptor-Like Channel 2
Vanilloid Receptor-Like Protein 2
Vrl-2
Vanilloid Receptor-Related Osmotically-Activated Channel
Vr-Oac
Database Links Reactome: R-HSA-3295583
Cellular Localisation Cell Membrane
Apical Cell Membrane
Multi-Pass Membrane Protein
Cell Junction
Adherens Junction
Cell Projection
Cilium
Assembly Of The Putative Homotetramer Occurs Primarily In The Endoplasmic Reticulum
Localization To The Cell Membrane Is Inhibited By Wnk Kinases (Wnk1
Wnk2
Wnk3 Or Wnk4) In A Kinase-Independent Mechanism
Isoform 1: Cell Membrane
Isoform 5: Cell Membrane
Isoform 2: Endoplasmic Reticulum
Isoform 4: Endoplasmic Reticulum
Isoform 6: Endoplasmic Reticulum
Alternative Antibody Names Anti-Transient Receptor Potential Cation Channel Subfamily V Member 4 antibody
Anti-Trpv4 antibody
Anti-Osm-9-Like Trp Channel 4 antibody
Anti-Otrpc4 antibody
Anti-Transient Receptor Potential Protein 12 antibody
Anti-Trp12 antibody
Anti-Vanilloid Receptor-Like Channel 2 antibody
Anti-Vanilloid Receptor-Like Protein 2 antibody
Anti-Vrl-2 antibody
Anti-Vanilloid Receptor-Related Osmotically-Activated Channel antibody
Anti-Vr-Oac antibody
Anti-TRPV4 antibody
Anti-VRL2 antibody
Anti-VROAC antibody

Information sourced from Uniprot.org

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