| Host: |
NZ White Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Mouse/Human/Rat/Marmoset |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Nz White Rabbit polyclonal antibody anti-TRPV4 (300-400) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Shipped as lyophilised. Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material. |
| Purification: |
Ammonium sulphate precipitation |
| Dilution Range: |
A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other application. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
TRPV4 |
| Gene ID: |
59341 |
| Uniprot ID: |
TRPV4_HUMAN |
| Immunogen Region: |
300-400 |
| Specificity: |
Specific for TRPV4. |
| Immunogen: |
A synthetic peptide from aa region 300-400 of mouse TRPV4 conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in rat and human. |
| Post Translational Modifications | N-glycosylated. |
| Function | Non-selective calcium permeant cation channel involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by heat, low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers. Acts as a regulator of intracellular Ca(2+) in synoviocytes. Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8. Together with PKD2, forms mechano- and thermosensitive channels in cilium. Negatively regulates expression of PPARGC1A, UCP1, oxidative metabolism and respiration in adipocytes. Regulates expression of chemokines and cytokines related to pro-inflammatory pathway in adipocytes. Together with AQP5, controls regulatory volume decrease in salivary epithelial cells. Required for normal development and maintenance of bone and cartilage. In its inactive state, may sequester DDX3X at the plasma membrane. When activated, the interaction between both proteins is affected and DDX3X relocalizes to the nucleus. Isoform 5: Non-selective calcium permeant cation channel involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by phorbol esters. Has the same channel activity as isoform 1, and is activated by the same stimuli. Isoform 2: Lacks channel activity, due to impaired oligomerization and intracellular retention. Isoform 4: Lacks channel activity, due to impaired oligomerization and intracellular retention. Isoform 6: Lacks channel activity, due to impaired oligomerization and intracellular retention. (Microbial infection) Facilitates hepatitis C virus (HCV) replication, possibly through its action on DDX3X. (Microbial infection) Facilitates Dengue virus (DENV) replication, possibly through its action on DDX3X. (Microbial infection) Facilitates Zika virus (ZIKV) replication, possibly through its action on DDX3X. |
| Protein Name | Transient Receptor Potential Cation Channel Subfamily V Member 4Trpv4Osm-9-Like Trp Channel 4Otrpc4Transient Receptor Potential Protein 12Trp12Vanilloid Receptor-Like Channel 2Vanilloid Receptor-Like Protein 2Vrl-2Vanilloid Receptor-Related Osmotically-Activated ChannelVr-Oac |
| Database Links | Reactome: R-HSA-3295583 |
| Cellular Localisation | Cell MembraneApical Cell MembraneMulti-Pass Membrane ProteinCell JunctionAdherens JunctionCell ProjectionCiliumAssembly Of The Putative Homotetramer Occurs Primarily In The Endoplasmic ReticulumLocalization To The Cell Membrane Is Inhibited By Wnk Kinases (Wnk1Wnk2Wnk3 Or Wnk4) In A Kinase-Independent MechanismIsoform 1: Cell MembraneIsoform 5: Cell MembraneIsoform 2: Endoplasmic ReticulumIsoform 4: Endoplasmic ReticulumIsoform 6: Endoplasmic Reticulum |
| Alternative Antibody Names | Anti-Transient Receptor Potential Cation Channel Subfamily V Member 4 antibodyAnti-Trpv4 antibodyAnti-Osm-9-Like Trp Channel 4 antibodyAnti-Otrpc4 antibodyAnti-Transient Receptor Potential Protein 12 antibodyAnti-Trp12 antibodyAnti-Vanilloid Receptor-Like Channel 2 antibodyAnti-Vanilloid Receptor-Like Protein 2 antibodyAnti-Vrl-2 antibodyAnti-Vanilloid Receptor-Related Osmotically-Activated Channel antibodyAnti-Vr-Oac antibodyAnti-TRPV4 antibodyAnti-VRL2 antibodyAnti-VROAC antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
