• Immunofluorescence analysis of U-2 OS cells using TPI1 antibody (STJ118179) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded mouse kidney using TPI1 antibody (STJ118179) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded rat kidney using TPI1 antibody (STJ118179) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human lung cancer using TPI1 antibody (STJ118179) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using TPI1 antibody (STJ118179) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.

Anti-TPI1 antibody (STJ118179)

SKU:
STJ118179

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Host: Rabbit
Applications: WB, IHC, IF
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal anti-TPI1 antibody is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
IHC: 1:100-1:200
IF: 1:50-1:200
Uniprot ID: TPIS_HUMAN
Gene ID: 7167
Gene Symbol: TPI1
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-249 of human TPI1 (NP_000356.1).
Function Triosephosphate isomerase is an extremely efficient metabolic enzyme that catalyzes the interconversion between dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde-3-phosphate (G3P) in glycolysis and gluconeogenesis. It is also responsible for the non-negligible production of methylglyoxal a reactive cytotoxic side-product that modifies and can alter proteins, DNA and lipids.
Protein Name Triosephosphate Isomerase
Tim
Methylglyoxal Synthase
Triose-Phosphate Isomerase
Database Links Reactome: R-HSA-70171
Reactome: R-HSA-70263
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Triosephosphate Isomerase antibody
Anti-Tim antibody
Anti-Methylglyoxal Synthase antibody
Anti-Triose-Phosphate Isomerase antibody
Anti-TPI1 antibody
Anti-TPI antibody

Information sourced from Uniprot.org

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