• Immunohistochemistry analysis of paraffin-embedded mouse brain using TMOD3 antibody (STJ25880) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human esophagus using TMOD3 antibody (STJ25880) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using TMOD3 antibody (STJ25880) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-TMOD3 antibody (1-352) (STJ25880)

SKU:
STJ25880

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TMOD3 (1-352) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TMOD3
Gene ID: 29766
Uniprot ID: TMOD3_HUMAN
Immunogen Region: 1-352
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-352 of human TMOD3 (NP_055362.1).
Immunogen Sequence: MALPFRKDLEKYKDLDEDEL LGNLSETELKQLETVLDDLD PENALLPAGFRQKNQTSKST TGPFDREHLLSYLEKEALEH KDREDYVPYTGEKKGKIFIP KQKPVQTFTEEKVSLDPELE EALTSASDTELCDLAAILGM HNLITNTKFCNIMGSSNGVD QEHFSNVVKGEKILPVFDEP PNPTNVEESLKRTKENDAHL VEVNLNNIKNIPIPTLKDFA KALETNTHVKCFSLAATRS
Tissue Specificity Ubiquitous.
Function Blocks the elongation and depolymerization of the actin filaments at the pointed end. The Tmod/TM complex contributes to the formation of the short actin protofilament, which in turn defines the geometry of the membrane skeleton.
Protein Name Tropomodulin-3
Ubiquitous Tropomodulin
U-Tmod
Database Links Reactome: R-HSA-390522
Reactome: R-HSA-9013418
Reactome: R-HSA-9696264
Cellular Localisation Cytoplasm
Cytoskeleton
Alternative Antibody Names Anti-Tropomodulin-3 antibody
Anti-Ubiquitous Tropomodulin antibody
Anti-U-Tmod antibody
Anti-TMOD3 antibody

Information sourced from Uniprot.org

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