| Host: |
Mouse |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-E3 ubiquitin-protein ligase TRIM33 is suitable for use in Western Blot research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
3H7-C8-B9 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG2a |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
TRIM33 |
| Gene ID: |
51592 |
| Uniprot ID: |
TRI33_HUMAN |
| Specificity: |
This antibody detects endogenous levels of TIF1 gamma/TRIM33 and does not cross-react with related proteins. |
| Immunogen: |
Purified recombinant human TIF1 gamma/TRIM33 protein fragments expressed in E.coli. |
| Post Translational Modifications | Sumoylated with SUMO1. |
| Function | Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor. Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade). |
| Protein Name | E3 Ubiquitin-Protein Ligase Trim33Ectodermin HomologRet-Fused Gene 7 ProteinProtein Rfg7Ring-Type E3 Ubiquitin Transferase Trim33Transcription Intermediary Factor 1-GammaTif1-GammaTripartite Motif-Containing Protein 33 |
| Database Links | Reactome: R-HSA-2173795Reactome: R-HSA-9754189 |
| Cellular Localisation | NucleusIn Discrete Nuclear Dots Resembling Nuclear BodiesLocalizes To Sites Of Dna Damage |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Trim33 antibodyAnti-Ectodermin Homolog antibodyAnti-Ret-Fused Gene 7 Protein antibodyAnti-Protein Rfg7 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Trim33 antibodyAnti-Transcription Intermediary Factor 1-Gamma antibodyAnti-Tif1-Gamma antibodyAnti-Tripartite Motif-Containing Protein 33 antibodyAnti-TRIM33 antibodyAnti-KIAA1113 antibodyAnti-RFG7 antibodyAnti-TIF1G antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
