• Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:400 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:400 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:400 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Western blot analysis of CACO2 lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-TIE2 antibody (STJ99610)

SKU:
STJ99610

Current Stock:
Host: Rabbit
Applications: WB, IHC-P, ELISA
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal anti-TIE2 antibody is suitable for use in Western Blot, Immunohistochemistry and ELISA.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG
Dilution Range: IHC-p 100-300
WB 1:500-2000
ELISA 1:10000-20000
Specificity: This antibody detects endogenous levels of TIE2.
Uniprot ID: TIE2_HUMAN
Gene ID: 7010
Gene Symbol: TEK
Immunogen: Synthesized peptide derived from human TIE2.
Post Translational Modifications Proteolytic processing leads to the shedding of the extracellular domain (soluble TIE-2 alias sTIE-2). Autophosphorylated on tyrosine residues in response to ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Autophosphorylation occurs in a sequential manner, where Tyr-992 in the kinase activation loop is phosphorylated first, followed by autophosphorylation at Tyr-1108 and at additional tyrosine residues. ANGPT1-induced phosphorylation is impaired during hypoxia, due to increased expression of ANGPT2. Phosphorylation is important for interaction with GRB14, PIK3R1 and PTPN11. Phosphorylation at Tyr-1102 is important for interaction with SHC1, GRB2 and GRB7. Phosphorylation at Tyr-1108 is important for interaction with DOK2 and for coupling to downstream signal transduction pathways in endothelial cells. Dephosphorylated by PTPRB. Ubiquitinated. The phosphorylated receptor is ubiquitinated and internalized, leading to its degradation.
Function Tyrosine-protein kinase that acts as cell-surface receptor for ANGPT1, ANGPT2 and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Has anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels. Required for normal angiogenesis and heart development during embryogenesis. Required for post-natal hematopoiesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. ANGPT1 signaling triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Signaling is modulated by ANGPT2 that has lower affinity for TEK, can promote TEK autophosphorylation in the absence of ANGPT1, but inhibits ANGPT1-mediated signaling by competing for the same binding site. Signaling is also modulated by formation of heterodimers with TIE1, and by proteolytic processing that gives rise to a soluble TEK extracellular domain. The soluble extracellular domain modulates signaling by functioning as decoy receptor for angiopoietins. TEK phosphorylates DOK2, GRB7, GRB14, PIK3R1.SHC1 and TIE1.
Protein Name Angiopoietin-1 Receptor
Endothelial Tyrosine Kinase
Tunica Interna Endothelial Cell Kinase
Tyrosine Kinase With Ig And Egf Homology Domains-2
Tyrosine-Protein Kinase Receptor Tek
Tyrosine-Protein Kinase Receptor Tie-2
Htie2
P140 Tek
Cd Antigen Cd202b
Database Links Reactome: R-HSA-210993
Reactome: R-HSA-5673001
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Cell Junction
Focal Adhesion
Cytoplasm
Cytoskeleton
Secreted
Recruited To Cell-Cell Contacts In Quiescent Endothelial Cells
Colocalizes With The Actin Cytoskeleton And At Actin Stress Fibers During Cell Spreading
Recruited To The Lower Surface Of Migrating Cells
Especially The Rear End Of The Cell
Proteolytic Processing Gives Rise To A Soluble Extracellular Domain That Is Secreted
Alternative Antibody Names Anti-Angiopoietin-1 Receptor antibody
Anti-Endothelial Tyrosine Kinase antibody
Anti-Tunica Interna Endothelial Cell Kinase antibody
Anti-Tyrosine Kinase With Ig And Egf Homology Domains-2 antibody
Anti-Tyrosine-Protein Kinase Receptor Tek antibody
Anti-Tyrosine-Protein Kinase Receptor Tie-2 antibody
Anti-Htie2 antibody
Anti-P140 Tek antibody
Anti-Cd Antigen Cd202b antibody
Anti-TEK antibody
Anti-TIE2 antibody
Anti-VMCM antibody
Anti-VMCM1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance