• STJ73468 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing nuclear and cytoplasmic staining. The nuclear stai
  • STJ73468 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4ug/ml). IgG control: Unimmunized goat IgG (black line)
  • STJ73468 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing nuclear and cytoplasmic staining. The nuclear stai
  • STJ73468 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4ug/ml). IgG control: Unimmunized goat IgG (black line)

Anti-TIAM1 Antibody (STJ73468)

SKU:
STJ73468

Current Stock:
Host: Goat
Applications: Pep-ELISA, IF, FC
Reactivity: Human
Short Description: Goat polyclonal anti-TIAM1 antibody is suitable for use in ELISA, Immunofluorescence and Flow Cytometry.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at-20°C. Minimize freezing and thawing.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage Instruction: Store at-20°C and avoid freeze-thaw cycles.
Concentration: 0.5mg/mL
Isotype: IgG
Dilution Range: IF-Strong expression of the protein seen in the cytoplasm and nuclei of U2OS cells. 10µg/ml
FC-Flow cytometric analysis of Jurkat cells. 10ug/ml
ELISA-antibody detection limit dilution 1:128000.
Immunogen Region: C Terminus
Immunogen Sequence: EDFAPSRKLNTEI
Accession Number: NP_003244; NP_001340613.1; NP_001340614.1; NP_001340615.1; NP_001340616.1
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance