• Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using TIAM1 antibody (STJ112290) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using TIAM1 antibody (STJ112290) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of Rat testis, using TIAM1 antibody (STJ112290) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-TIAM1 antibody (1392-1591) (STJ112290)

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STJ112290

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TIAM1 (1392-1591) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TIAM1
Gene ID: 7074
Uniprot ID: TIAM1_HUMAN
Immunogen Region: 1392-1591
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1392-1591 of human TIAM1 (NP_003244.2).
Immunogen Sequence: SILRDKHRRQLLKTESLPSS QQYVPFGGKRLCALKGARPA MSRAVSAPSKSLGRRRRRLA RNRFTIDSDAVSASSPEKES QQPPGGGDTDRWVEEQFDLA QYEEQDDIKETDILSDDDEF CESVKGASVDRDLQERLQAT SISQRERGRKTLDSHASRMA QLKKQAALSGINGGLESASE EVIWVRREDFAPSRKLNTEI
Tissue Specificity Found in virtually all analyzed tumor cell lines including B- and T-lymphomas, neuroblastomas, melanomas and carcinomas.
Post Translational Modifications Ubiquitinated. Undergoes 'Lys-48' ubiquitination at Lys-1404 and Lys-1420 by a CUL3(KBTBD6/7) E3 ubiquitin ligase complex composed of CUL3, RBX1, KBTBD6 and KBTBD7. 'Lys-48' ubiquitination at Lys-1404 and Lys-1420 triggers proteasomal degradation. Ubiquitination at Lys-1404 and Lys-1420 by CUL3(KBTBD6/7) also requires the membrane-associated protein GABARAP and may therefore be spatially restricted within the cell.
Function Guanyl-nucleotide exchange factor that activates RHO-like proteins and connects extracellular signals to cytoskeletal activities. Activates RAC1, CDC42, and to a lesser extent RHOA and their downstream signaling to regulate processes like cell adhesion and cell migration.
Protein Name Rho Guanine Nucleotide Exchange Factor Tiam1
T-Lymphoma Invasion And Metastasis-Inducing Protein 1
Tiam-1
Database Links Reactome: R-HSA-193648
Reactome: R-HSA-3928662
Reactome: R-HSA-3928665
Reactome: R-HSA-416482
Reactome: R-HSA-8980692
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Reactome: R-HSA-9013423
Reactome: R-HSA-9032845
Cellular Localisation Cell Junction
Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Detected At The Boundary Between Cells With Actin-Rich Protrusions
Presence Of Krit1
Cdh5 And Rap1b Is Required For Its Localization To The Cell Junction
Alternative Antibody Names Anti-Rho Guanine Nucleotide Exchange Factor Tiam1 antibody
Anti-T-Lymphoma Invasion And Metastasis-Inducing Protein 1 antibody
Anti-Tiam-1 antibody
Anti-TIAM1 antibody

Information sourced from Uniprot.org

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