• Immunofluorescence analysis of mouse heart using Desmin Rabbit mAb (STJ11101864) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded human colon using Desmin Rabbit mAb (STJ11101864) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of rat heart using Desmin Rabbit mAb (STJ11101864) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using Desmin Rabbit mAb (STJ11101864) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.

Anti-Thyroglobulin antibody [ARC1971] (STJ11101864)

SKU:
STJ11101864

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Host: Rabbit
Applications: IHC, IF
Reactivity: Human, Mouse, Rat
Short Description: Rabbit monoclonal anti-Thyroglobulin antibody is suitable for use in Immunohistochemistry and Immunofluorescence.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Monoclonal
Clone ID: ARC1971
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: IHC: 1:50-1:200
IF: 1:50-1:200
Uniprot ID: THYG_HUMAN
Gene ID: 7038
Gene Symbol: TG
Immunogen: A synthesized peptide derived from human Thyroglobulin
Tissue Specificity Specifically expressed in the thyroid gland.
Post Translational Modifications Iodinated on tyrosine residues by TPO. There are 4 pairs of iodinated tyrosines used for coupling: acceptor Tyr-24 is coupled to donor Tyr-149 or Tyr-234, acceptor Tyr-2573 is coupled to donor Tyr-2540, acceptor Tyr-2766 in monomer 1 is coupled to donor Tyr-2766 in monomer 2 and acceptor Tyr-1310 in monomer 1 is coupled to donor Tyr-108 in monomer 2. Sulfated tyrosines are desulfated during iodination. Undergoes sequential proteolysis by cathepsins to release thyroxine (T4) and triiodothyronine (T3) hormones. In the thyroid follicle lumen, cross-linked TG (storage form) is solubilized by limited proteolysis mediated by cathepsins CTSB and/or CTSL. Partially cleaved TG is further processed by CTSK/cathepsin K and/or CTSL resulting in the release of T4. Following endocytosis, further processing occurs leading to the release of T3 and more T4 hormones.
Function Acts as a substrate for the production of iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). The synthesis of T3 and T4 involves iodination of selected tyrosine residues of TG/thyroglobulin followed by their oxidative coupling in the thyroid follicle lumen. Following TG re-internalization and lysosomal-mediated proteolysis, T3 and T4 are released from the polypeptide backbone leading to their secretion into the bloodstream. One dimer produces 7 thyroid hormone molecules.
Protein Name Thyroglobulin
Tg
Cellular Localisation Secreted
Secreted Into The Thyroid Follicle Lumen
Localizes To Colloid Globules
A Structure Formed In The Thyroid Follicle Lumen Consisting Of Cross-Linked Tg Arranged In Concentric Layers
Alternative Antibody Names Anti-Thyroglobulin antibody
Anti-Tg antibody
Anti-TG antibody

Information sourced from Uniprot.org

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