• Immunohistochemistry analysis of paraffin-embedded rat bone marrow using TET2 antibody (STJ25816) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using TET2 antibody (STJ25816) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of Mouse heart, using TET2 antibody (STJ25816) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-TET2 antibody (1833-2002) (STJ25816)

SKU:
STJ25816

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TET2 (1833-2002) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TET2
Gene ID: 54790
Uniprot ID: TET2_HUMAN
Immunogen Region: 1833-2002
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1833-2002 of human TET2 (NP_001120680.1).
Immunogen Sequence: VQGVASGAEDNDEVWSDSEQ SFLDPDIGGVAVAPTHGSIL IECAKRELHATTPLKNPNRN HPTRISLVFYQHKSMNEPKH GLALWEAKMAEKAREKEEEC EKYGPDYVPQKSHGKKVKRE PAEPHETSEPTYLRFIKSLA ERTMSVTTDSTVTTSPYAFT RVTGPYNRYI
Tissue Specificity Broadly expressed. Highly expressed in hematopoietic cells.highest expression observed in granulocytes. Expression is reduced in granulocytes from peripheral blood of patients affected by myelodysplastic syndromes.
Post Translational Modifications May be glycosylated. It is unclear whether interaction with OGT leads to GlcNAcylation. According to a report, it is not GlcNAcylated by OGT. In contrast, another group reports GlcNAcylation by OGT in mouse ortholog. Monoubiquitinated at Lys-1299 by the DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex called CRL4(VprBP) or CUL4A-RBX1-DDB1-DCAF1/VPRBP complex.this modification promotes binding to DNA.
Function Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation. Has a preference for 5-hydroxymethylcytosine in CpG motifs. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT.
Protein Name Methylcytosine Dioxygenase Tet2
Database Links Reactome: R-HSA-5221030
Reactome: R-HSA-9827857
Cellular Localisation Nucleus
Chromosome
Localization To Chromatin Depends Upon Monoubiquitination At Lys-1299
Alternative Antibody Names Anti-Methylcytosine Dioxygenase Tet2 antibody
Anti-TET2 antibody
Anti-KIAA1546 antibody
Anti-Nbla00191 antibody

Information sourced from Uniprot.org

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