• Western blot analysis of extracts of various cell lines, using TERF1 antibody (STJ27246) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-TERF1-antibody (aa240-419) (STJ27246)


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Host: Rabbit
Applications: WB, IF
Reactivity: Human, Mouse
Short Description: Rabbit polyclonal antibody anti-TERF1 is suitable for use in Western Blot and Immunofluorescence.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF 1:50-1:200
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Gene Symbol: TERF1
Gene ID: 7013
Immunogen Region: 240-419
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 240-419 of human TERF1 (NP_003209.2).
Tissue Specificity Highly expressed and ubiquitous. Isoform Pin2 predominates.
Post Translational Modifications Phosphorylated preferentially on Ser-219 in an ATM-dependent manner in response to ionizing DNA damage. ADP-ribosylation by TNKS1 or TNKS2 diminishes its ability to bind to telomeric DNA. Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex, leading to its degradation by the proteasome.
Function Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends.without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.
Protein Name Telomeric Repeat-Binding Factor 1
Nima-Interacting Protein 2
Ttaggg Repeat-Binding Factor 1
Telomeric Protein Pin2/Trf1
Database Links Reactome: R-HSA-110328
Reactome: R-HSA-110329
Reactome: R-HSA-110330
Reactome: R-HSA-110331
Reactome: R-HSA-1221632
Reactome: R-HSA-171306
Reactome: R-HSA-171319
Reactome: R-HSA-174411
Reactome: R-HSA-174414
Reactome: R-HSA-174417
Reactome: R-HSA-174430
Reactome: R-HSA-174437
Reactome: R-HSA-2559586
Reactome: R-HSA-9670095
Cellular Localisation Nucleus
Colocalizes With Telomeric Dna In Interphase And Prophase Cells
Telomeric Localization Decreases In Metaphase
Anaphase And Telophase
Associates With The Mitotic Spindle
Alternative Antibody Names Anti-Telomeric Repeat-Binding Factor 1 antibody
Anti-Nima-Interacting Protein 2 antibody
Anti-Ttaggg Repeat-Binding Factor 1 antibody
Anti-Telomeric Protein Pin2/Trf1 antibody
Anti-TERF1 antibody
Anti-PIN2 antibody
Anti-TRBF1 antibody
Anti-TRF antibody
Anti-TRF1 antibody

Information sourced from Uniprot.org

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