• Immunofluorescence analysis of HeLa cells using TARS antibody (STJ29073). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using TARS antibody (STJ29073) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat heart using TARS antibody (STJ29073) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using TARS antibody (STJ29073) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using TARS Antibody (STJ29073) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-TARS antibody (1-230) (STJ29073)

SKU:
STJ29073

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TARS (1-230) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TARS1
Gene ID: 6897
Uniprot ID: SYTC_HUMAN
Immunogen Region: 1-230
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human TARS (NP_689508.3).
Immunogen Sequence: MFEEKASSPSGKMGGEEKPI GAGEEKQKEGGKKKNKEGSG DGGRAELNPWPEYIYTRLEM YNILKAEHDSILAEKAEKDS KPIKVTLPDGKQVDAESWKT TPYQIACGISQGLADNTVIA KVNNVVWDLDRPLEEDCTLE LLKFEDEEAQAVYWHSSAHI MGEAMERVYGGCLCYGPPIE NGFYYDMYLEEGGVSSNDFS SLEALCKKIIKEKQAFERLE VKKETLLAMF
Post Translational Modifications ISGylated.
Function Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction: threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged tRNA(Thr) via its editing domain, at the post-transfer stage.
Protein Name Threonine--Trna Ligase 1 - Cytoplasmic
Threonyl-Trna Synthetase
Thrrs
Threonyl-Trna Synthetase 1
Database Links Reactome: R-HSA-379716
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Threonine--Trna Ligase 1 - Cytoplasmic antibody
Anti-Threonyl-Trna Synthetase antibody
Anti-Thrrs antibody
Anti-Threonyl-Trna Synthetase 1 antibody
Anti-TARS1 antibody
Anti-TARS antibody

Information sourced from Uniprot.org

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