• Immunoprecipitation analysis of 100 Mu g extracts of HepG2 cells using 3 Mu g SQSTM1/p62 antibody (STJ113162). Western blot was performed from the immunoprecipitate using SQSTM1/p62 antibody (STJ113162) at a dilution of 1:1000.
  • Immunofluorescence analysis of U2OS cells using SQSTM1/p62 Rabbit polyclonal antibody (STJ113162) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of SQSTM1/p62 in paraffin-embedded Mouse liver using SQSTM1/p62 Rabbit polyclonal antibody (STJ113162) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SQSTM1/p62 in paraffin-embedded Human appendix using SQSTM1/p62 Rabbit polyclonal antibody (STJ113162) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SQSTM1/p62 in paraffin-embedded Rat ovary using SQSTM1/p62 Rabbit polyclonal antibody (STJ113162) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using SQSTM1/p62 Rabbit polyclonal antibody (STJ113162) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SQSTM1 antibody (1-440) (STJ113162)

SKU:
STJ113162

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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SQSTM1/p62 (1-440) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
IP 1:20-1:50
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SQSTM1
Gene ID: 8878
Uniprot ID: SQSTM_HUMAN
Immunogen Region: 1-440
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-440 of human SQSTM1/p62 (NP_003891.1).
Immunogen Sequence: MASLTVKAYLLGKEDAAREI RRFSFCCSPEPEAEAEAAAG PGPCERLLSRVAALFPALRP GGFQAHYRDEDGDLVAFSSD EELTMAMSYVKDDIFRIYIK EKKECRRDHRPPCAQEAPRN MVHPNVICDGCNGPVVGTRY KCSVCPDYDLCSVCEGKGLH RGHTKLAFPSPFGHLSEGFS HSRWLRKVKHGHFGWPGWEM GPPGNWSPRPPRAGEARPGP TAESASGPSEDPSVNFLKN
Tissue Specificity Ubiquitously expressed.
Post Translational Modifications Phosphorylated. May be phosphorylated by PRKCZ. Phosphorylated in vitro by TTN. Phosphorylation at Ser-403 by ULK1 is stimulated by SESN2. Phosphorylated at Ser-403 by TBK1, leading to promote relocalization of 'Lys-63'-linked ubiquitinated STING1 to autophagosomes. Phosphorylation at Ser-349 by MTOR promotes interaction with KEAP1 and inactivation of the BCR(KEAP1) complex, promoting NFE2L2/NRF2 nuclear accumulation and expression of phase II detoxifying enzymes. Ubiquitinated by UBE2J1 and RNF26 at Lys-435: ubiquitinated SQSTM1 attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transport. Deubiquitination by USP15 releases target vesicles for fast transport into the cell periphery. Ubiquitinated by the BCR(KEAP1) complex at Lys-420, increasing SQSTM1 sequestering activity and promoting its degradation. Ubiquitinated via 'Lys-29' and 'Lys-33'-linked polyubiquitination leading to xenophagic targeting of bacteria and inhibition of their replication. (Microbial infection) Cleaved by S.pyogenes SpeB protease.leading to its degradation. Degradation by SpeB prevents autophagy, promoting to S.pyogenes intracellular replication. (Microbial infection) Deubiquitinated by Epstein-Barr virus BPLF1.leading to inhibition of the recruitment of MAP1LC3A/LC3 to SQSTM1-positive structures.
Function Autophagy receptor required for selective macroautophagy (aggrephagy). Functions as a bridge between polyubiquitinated cargo and autophagosomes. Interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family. Along with WDFY3, involved in the formation and autophagic degradation of cytoplasmic ubiquitin-containing inclusions (p62 bodies, ALIS/aggresome-like induced structures). Along with WDFY3, required to recruit ubiquitinated proteins to PML bodies in the nucleus. Also involved in autophagy of peroxisomes (pexophagy) in response to reactive oxygen species (ROS) by acting as a bridge between ubiquitinated PEX5 receptor and autophagosomes. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD. May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. Involved in endosome organization by retaining vesicles in the perinuclear cloud: following ubiquitination by RNF26, attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transport. Promotes relocalization of 'Lys-63'-linked ubiquitinated STING1 to autophagosomes. Acts as an activator of the NFE2L2/NRF2 pathway via interaction with KEAP1: interaction inactivates the BCR(KEAP1) complex, promoting nuclear accumulation of NFE2L2/NRF2 and subsequent expression of cytoprotective genes. Sequesters tensin TNS2 into cytoplasmic puncta, promoting TNS2 ubiquitination and proteasomal degradation.
Protein Name Sequestosome-1
Ebi3-Associated Protein Of 60 Kda
Ebiap
P60
Phosphotyrosine-Independent Ligand For The Lck Sh2 Domain Of 62 Kda
Ubiquitin-Binding Protein P62
Database Links Reactome: R-HSA-205043
Reactome: R-HSA-209543
Reactome: R-HSA-209560
Reactome: R-HSA-5205685
Reactome: R-HSA-8951664
Reactome: R-HSA-9020702
Reactome: R-HSA-9664873
Reactome: R-HSA-9725370
Reactome: R-HSA-9755511
Reactome: R-HSA-9759194
Cellular Localisation Cytoplasm
Cytosol
Preautophagosomal Structure
Late Endosome
Lysosome
Cytoplasmic Vesicle
Autophagosome
Nucleus
Endoplasmic Reticulum
Pml Body
Myofibril
Sarcomere
In Cardiac Muscle
Localizes To The Sarcomeric Band
Commonly Found In Inclusion Bodies Containing Polyubiquitinated Protein Aggregates
In Neurodegenerative Diseases
Detected In Lewy Bodies In Parkinson Disease
Neurofibrillary Tangles In Alzheimer Disease
And Htt Aggregates In Huntington Disease
In Protein Aggregate Diseases Of The Liver
Found In Large Amounts In Mallory Bodies Of Alcoholic And Nonalcoholic Steatohepatitis
Hyaline Bodies In Hepatocellular Carcinoma
And In Serpina1 Aggregates
Enriched In Rosenthal Fibers Of Pilocytic Astrocytoma
In The Cytoplasm
Observed In Both Membrane-Free Ubiquitin-Containing Protein Aggregates (Sequestosomes) And Membrane-Surrounded Autophagosomes
Colocalizes With Trim13 In The Perinuclear Endoplasmic Reticulum
Co-Localizes With Trim5 In Cytoplasmic Bodies
When Nuclear Export Is Blocked By Treatment With Leptomycin B
Accumulates In Pml Bodies
Alternative Antibody Names Anti-Sequestosome-1 antibody
Anti-Ebi3-Associated Protein Of 60 Kda antibody
Anti-Ebiap antibody
Anti-P60 antibody
Anti-Phosphotyrosine-Independent Ligand For The Lck Sh2 Domain Of 62 Kda antibody
Anti-Ubiquitin-Binding Protein P62 antibody
Anti-SQSTM1 antibody
Anti-ORCA antibody
Anti-OSIL antibody

Information sourced from Uniprot.org

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