||NZ White Rabbit
||Human, Mouse, Rat
||NZ White Rabbit polyclonal anti-Spinophilin antibody is suitable for use in Immunohistochemistry and Western Blot.
||FOR RESEARCH USE ONLY (RUO).
||Shipped as lyophilised. Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
||Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
||A dilution of 1:1000 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
||A synthetic peptide from aa region 450-500 of human Spinophilin conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse and rat.
| Function || Seems to act as a scaffold protein in multiple signaling pathways. Modulates excitatory synaptic transmission and dendritic spine morphology. Binds to actin filaments (F-actin) and shows cross-linking activity. Binds along the sides of the F-actin. May play an important role in linking the actin cytoskeleton to the plasma membrane at the synaptic junction. Believed to target protein phosphatase 1/PP1 to dendritic spines, which are rich in F-actin, and regulates its specificity toward ion channels and other substrates, such as AMPA-type and NMDA-type glutamate receptors. Plays a role in regulation of G-protein coupled receptor signaling, including dopamine D2 receptors and alpha-adrenergic receptors. May establish a signaling complex for dopaminergic neurotransmission through D2 receptors by linking receptors downstream signaling molecules and the actin cytoskeleton. Binds to ADRA1B and RGS2 and mediates regulation of ADRA1B signaling. May confer to Rac signaling specificity by binding to both, RacGEFs and Rac effector proteins. Probably regulates p70 S6 kinase activity by forming a complex with TIAM1. Required for hepatocyte growth factor (HGF)-induced cell migration. |
| Protein Name || Neurabin-2Neurabin-IiProtein Phosphatase 1 Regulatory Subunit 9bSpinophilin |
| Cellular Localisation || CytoplasmCytoskeletonNucleusCell ProjectionDendritic SpineCell JunctionSynapsePostsynaptic DensityAdherens JunctionCell MembraneLamellipodiumFilopodiumRuffle MembraneEnriched At Synapse And Cadherin-Based Cell-Cell Adhesion SitesIn NeuronsBoth Cytosolic And Membrane-AssociatedAnd Highly Enriched In The Postsynaptic Density Apposed To Exitatory SynapsesColocalizes With Ppp1r2 At Actin-Rich Adherens Junctions In Epithelial Cells And In Dendritic SpinesAccumulates In The LamellipodiumFilopodium And Ruffle Membrane In Response To Hepatocyte Growth Factor (Hgf) Treatment |
| Alternative Antibody Names || Anti-Neurabin-2 antibodyAnti-Neurabin-Ii antibodyAnti-Protein Phosphatase 1 Regulatory Subunit 9b antibodyAnti-Spinophilin antibodyAnti-PPP1R9B antibodyAnti-PPP1R6 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance