• Immunofluorescence analysis of HeLa cells using U1A/U1A/SNRPA antibody (STJ28493).
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using U1A/U1A/SNRPA Antibody (STJ28493) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human adenomyosis using U1A/U1A/SNRPA Antibody (STJ28493) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat heart using U1A/U1A/SNRPA Antibody (STJ28493) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using U1A/U1A/SNRPA antibody (STJ28493) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SNRPA antibody (1-282) (STJ28493)

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STJ28493

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SNRPA (1-282) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SNRPA
Gene ID: 6626
Uniprot ID: SNRPA_HUMAN
Immunogen Region: 1-282
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-282 of human U1A/U1A/SNRPA (NP_004587.1).
Immunogen Sequence: MAVPETRPNHTIYINNLNEK IKKDELKKSLYAIFSQFGQI LDILVSRSLKMRGQAFVIFK EVSSATNALRSMQGFPFYDK PMRIQYAKTDSDIIAKMKGT FVERDRKREKRKPKSQETPA TKKAVQGGGATPVVGAVQGP VPGMPPMTQAPRIMHHMPGQ PPYMPPPGMIPPPGLAPGQI PPGAMPPQQLMPGQMPPAQP LSENPPNHILFLTNLPEETN ELMLSMLFNQFPGFKEVRL
Function Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. U1 snRNP is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. SNRPA binds stem loop II of U1 snRNA. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process. May bind preferentially to the 5'-UGCAC-3' motif on RNAs.
Protein Name U1 Small Nuclear Ribonucleoprotein A
U1 Snrnp A
U1-A
U1a
Database Links Reactome: R-HSA-72163
Cellular Localisation Nucleus
Alternative Antibody Names Anti-U1 Small Nuclear Ribonucleoprotein A antibody
Anti-U1 Snrnp A antibody
Anti-U1-A antibody
Anti-U1a antibody
Anti-SNRPA antibody

Information sourced from Uniprot.org

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