• Western blot analysis of HeLa, using SMYD4 rabbit polyclonal antibody (STJ29449) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunohistochemistry analysis of paraffin-embedded rat spleen using SMYD4 antibody (STJ29449) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using SMYD4 antibody (STJ29449) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse lung using SMYD4 antibody (STJ29449) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of A-549 cells using SMYD4 antibody (STJ29449). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug SMYD4 rabbit polyclonal antibody (STJ29449 1:2000). Western blot was performed from the immunoprecipitate using SMYD4 rabbit polyclonal antibody (STJ29449) at a dilition of 1:1000.

Anti-SMYD4 antibody (1-260) (STJ29449)

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STJ29449

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SMYD4 (1-260) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SMYD4
Gene ID: 114826
Uniprot ID: SMYD4_HUMAN
Immunogen Region: 1-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human SMYD4 (NP_443160.2).
Immunogen Sequence: MDLPVDEWKSYLLQKWASLP TSVQVTISTAETLRDIFLHS SSLLQPEDELFLKRLSKGYL VGKDSDAPLFYREEGNKKFQ EKDYTGAAVLYSKGVSHSRP NTEDMSLCHANRSAALFHLG QYETCLKDINRAQTHGYPER LQPKIMLRKAECLVALGRLQ EASQTISDLERNFTATPALA DVLPQTLQRNLHRLKMKMQE KDSLTESFPAALAKTLEDAA LREENEQLSNASSSIGLCV
Function Plays a critical role in cardiac development. Acts as a key epigenetic regulator of gene expression during cardiac development via its dual activities as a methyltransferase and negative regulator of HDAC1.
Protein Name Set And Mynd Domain-Containing Protein 4
Cellular Localisation Nucleus
Cytoplasm
Alternative Antibody Names Anti-Set And Mynd Domain-Containing Protein 4 antibody
Anti-SMYD4 antibody
Anti-KIAA1936 antibody

Information sourced from Uniprot.org

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