Anti-SLC4A4 antibody (978-1078) (STJ27285)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal SLC4A4/NBC (978-1078) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SLC4A4 |
| Gene ID | 8671 |
| Uniprot ID | S4A4_HUMAN |
| Immunogen Region | 978-1078 |
| Immunogen Sequence | VMILALVAVRKGMDYLFSQH DLSFLDDVIPEKDKKKKEDE KKKKKKKGSLDSDNDDSDCP YSEKVPSIKIPMDIMEQQPF LSDSKPSDRERSPTFLERHT S |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 978-1078 of human SLC4A4/NBC (NP_001091954.1). |
Additional Info
| Tissue Specificity | Isoform 1: Expressed in the corneal endothelium cells (at protein level). Expressed in pancreas and to a lower extent in heart, skeletal muscle, liver, parotid salivary glands, prostate, colon, stomach, thyroid, brain and spinal cord. Isoform 2: Specifically expressed in kidney at the level of proximal tubules. |
| Post Translational Modifications | Phosphorylation of Ser-1026 by PKA increases the binding of CA2 and changes the Na(+):HCO3(-) stoichiometry of the transporter from 3:1 to 2:1. Phosphorylated in presence of STK39 and dephosphorylated in presence of PP1 phosphatase.phosphorylation seems to inhibit SLC4A4 activity. N-glycosylated. May not be necessary for the transporter basic functions. |
| Function | Electrogenic sodium/bicarbonate cotransporter with a Na(+):HCO3(-) stoichiometry varying from 1:2 to 1:3. May regulate bicarbonate influx/efflux at the basolateral membrane of cells and regulate intracellular pH. |
| Protein Name | Electrogenic Sodium Bicarbonate Cotransporter 1Sodium Bicarbonate CotransporterNa(+/Hco3(- CotransporterSolute Carrier Family 4 Member 4Knbc1 |
| Database Links | Reactome: R-HSA-425381Reactome: R-HSA-5619054 |
| Cellular Localisation | Basolateral Cell MembraneMulti-Pass Membrane ProteinCell Membrane |
| Alternative Antibody Names | Anti-Electrogenic Sodium Bicarbonate Cotransporter 1 antibodyAnti-Sodium Bicarbonate Cotransporter antibodyAnti-Na(+/Hco3(- Cotransporter antibodyAnti-Solute Carrier Family 4 Member 4 antibodyAnti-Knbc1 antibodyAnti-SLC4A4 antibodyAnti-NBC antibodyAnti-NBC1 antibodyAnti-NBCE1 antibody |
Information sourced from Uniprot.org