• Western blot analysis of extracts of mouse pancreas, using SFRP2 antibody (STJ114825) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Immunofluorescence analysis of A431 cells using SFRP2 rabbit polyclonal antibody (STJ114825) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using SFRP2 rabbit polyclonal antibody (STJ114825) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using SFRP2 rabbit polyclonal antibody (STJ114825) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-SFRP2 antibody (1-100) (STJ114825)

SKU:
STJ114825

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SFRP2 (1-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:3000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SFRP2
Gene ID: 6423
Uniprot ID: SFRP2_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SFRP2 (NP_003004.1).
Immunogen Sequence: MLQGPGSLLLLFLASHCCLG SARGLFLFGQPDFSYKRSNC KPIPANLQLCHGIEYQNMRL PNLLGHETMKEVLEQAGAWI PLVMKQCHPDTKKFLCSLFA
Tissue Specificity Expressed in adipose tissue, heart, brain, skeletal muscle, pancreas, thymus, prostate, testis, ovary, small intestine and colon. Highest levels in adipose tissue, small intestine and colon.
Function Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis.
Protein Name Secreted Frizzled-Related Protein 2
Frp-2
Sfrp-2
Secreted Apoptosis-Related Protein 1
Sarp-1
Database Links Reactome: R-HSA-201681
Reactome: R-HSA-3772470
Cellular Localisation Secreted
Alternative Antibody Names Anti-Secreted Frizzled-Related Protein 2 antibody
Anti-Frp-2 antibody
Anti-Sfrp-2 antibody
Anti-Secreted Apoptosis-Related Protein 1 antibody
Anti-Sarp-1 antibody
Anti-SFRP2 antibody
Anti-FRP2 antibody
Anti-SARP1 antibody
Anti-FKSG12 antibody
Anti-UNQ361 antibody
Anti-PRO697 antibody

Information sourced from Uniprot.org

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