• Western blot analysis of extracts of various cell lines, using 14-3-3 sigma rabbit monoclonal antibody (STJ11102062) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Immunohistochemistry of paraffin-embedded human colon using 14-3-3 sigma rabbit monoclonal antibody (STJ11102062) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-SFN antibody (14-3) [ARC0988] (STJ11102062)


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Host: Rabbit
Applications: WB, IHC
Reactivity: Human, Mouse
Short Description: Rabbit monoclonal antibody anti-14-3-3 sigma (14-3) is suitable for use in Western Blot and Immunohistochemistry.
Clonality: Monoclonal
Clone ID: ARC0988
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS containing 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC 1:50-1:200
Storage Instruction: Store in a freezer at-20°C and avoid freeze-thaw cycles.
Gene Symbol: SFN
Gene ID: 2810
Uniprot ID: 1433S_HUMAN
Immunogen Region: 14-3
Immunogen: A synthesized peptide derived from human 14-3-3 sigma
Tissue Specificity Present mainly in tissues enriched in stratified squamous keratinizing epithelium.
Post Translational Modifications Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation.
Function Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53. p53-regulated inhibitor of G2/M progression.
Protein Name 14-3-3 Protein Sigma
Epithelial Cell Marker Protein 1
Database Links Reactome: R-HSA-111447
Reactome: R-HSA-1445148
Reactome: R-HSA-5625740
Reactome: R-HSA-5628897
Reactome: R-HSA-6804114
Reactome: R-HSA-75035
Reactome: R-HSA-9614399
Cellular Localisation Cytoplasm
May Be Secreted By A Non-Classical Secretory Pathway
Alternative Antibody Names Anti-14-3-3 Protein Sigma antibody
Anti-Epithelial Cell Marker Protein 1 antibody
Anti-Stratifin antibody
Anti-SFN antibody
Anti-HME1 antibody

Information sourced from Uniprot.org

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