• Western blot analysis of extracts of 293T cells, using SARS-CoV-2 Spike ECD antibody (STJ11103348) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of 293T cells, using SARS-CoV-2 Spike ECD antibody (STJ11103348) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-SARS-CoV-2-Spike ECD antibody (STJ11103348)

SKU:
STJ11103348
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: SARS-CoV-2
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit polyclonal antibody anti-SARS-CoV-2-Spike ECD is suitable for use in Western Blot.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS containing 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: ELISA 1:20000-1:80000
WB 1:500-1:2000
Storage Instruction: Store in a freezer at-20°C and avoid freeze-thaw cycles.
Immunogen: Recombinant fusion protein of SARS-CoV-2 Spike ECD.
Background The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates thisinteraction.The S protein plays key parts in the induction of neutralizing-antibody and T-cellresponses, as well as protective immunity.

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance