• Immunoprecipitation analysis of 200 Mu g extracts of THP-1 cells using 3 Mu g S100A11 antibody (STJ27439). Western blot was performed from the immunoprecipitate using S100A11 antibody (STJ27439) at a dilution of 1:1000.
  • Immunofluorescence analysis of U2OS cells using [KO Validated] S100A11 Rabbit polyclonal antibody (STJ27439). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from wild type (WT) and S100A11 knockout (KO) HeLa cells, using [KO Validated] S100A11 Rabbit polyclonal antibody (STJ27439) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of various lysates using [KO Validated] S100A11 Rabbit polyclonal antibody (STJ27439) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-S100A11 antibody (1-105) (STJ27439)

SKU:
STJ27439

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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-S100A11 (1-105) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: S100A11
Gene ID: 6282
Uniprot ID: S10AB_HUMAN
Immunogen Region: 1-105
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human S100A11 (NP_005611.1).
Immunogen Sequence: MAKISSPTETERCIESLIAV FQKYAGKDGYNYTLSKTEFL SFMNTELAAFTKNQKDPGVL DRMMKKLDTNSDGQLDFSEF LNLIGGLAMACHDSFLKAVP SQKRT
Post Translational Modifications Phosphorylation at Thr-10 by PRKCA significantly suppresses homodimerization and promotes association with NCL/nucleolin which induces nuclear translocation.
Function Facilitates the differentiation and the cornification of keratinocytes.
Protein Name Protein S100-A11
Calgizzarin
Metastatic Lymph Node Gene 70 Protein
Mln 70
Protein S100-C
S100 Calcium-Binding Protein A11 Cleaved Into - Protein S100-A11 - N-Terminally Processed
Database Links Reactome: R-HSA-6798695
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Protein S100-A11 antibody
Anti-Calgizzarin antibody
Anti-Metastatic Lymph Node Gene 70 Protein antibody
Anti-Mln 70 antibody
Anti-Protein S100-C antibody
Anti-S100 Calcium-Binding Protein A11 Cleaved Into - Protein S100-A11 - N-Terminally Processed antibody
Anti-S100A11 antibody
Anti-MLN70 antibody
Anti-S100C antibody

Information sourced from Uniprot.org

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