Anti-ROBO1 antibody (STJ191349)

SKU:
STJ191349
Current Stock:
Host: Rabbit
Applications: WB, ELISA
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal primary antibody against ROBO1.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Purification: ROBO1 antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG
Dilution Range: WB 1:500-2000
ELISA 1:5000-20000
Specificity: ROBO1 polyclonal antibody detects endogenous levels of protein.
Uniprot ID: ROBO1_HUMAN
Gene ID: 6091
Gene Symbol: ROBO1
Immunogen: Synthesized peptide derived from human ROBO1 protein.
Immunogen Region: 730-810aa
Tissue Specificity Widely expressed, with exception of kidney.
Post Translational Modifications Ubiquitinated. May be deubiquitinated by USP33.
Function Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. Plays a role in the regulation of cell migration via its interaction with MYO9B.inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA. May be required for lung development.
Database Links Reactome: R-HSA-373752
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Cell Projection
Axon
Endoplasmic Reticulum-Golgi Intermediate Compartment Membrane
Single-Pass Membrane Protein
Detected At Growth Cones In Thalamus Neurons
Prrg4 Prevents Cell Surface Location And Both Colocalize In The Endoplasmic Reticulum/Golgi Adjacent To The Cell Nucleus
Protein Name Roundabout Homolog 1
Deleted In U Twenty Twenty
H-Robo-1
Alternative Names Anti-Roundabout Homolog 1 antibody
Anti-Deleted In U Twenty Twenty antibody
Anti-H-Robo-1 antibody
Anti-ROBO1 antibody
Anti-DUTT1 antibody

Information sourced from Uniprot.org

6 months standard