• Western blot analysis of lysates from NIH/3T3 cells, using ReC overin Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of 3T3 cells using ReC overin Polyclonal Antibody

Anti-Recoverin antibody (STJ95402)

SKU:
STJ95402

Current Stock:
Host: Rabbit
Applications: WB, IHC-P, IF, ELISA
Reactivity: Human, Mouse
Short Description: Rabbit polyclonal anti-Recoverin antibody is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Specificity: Recoverin polyclonal antibody detects endogenous levels of Recoverin protein.
Uniprot ID: RECO_HUMAN
Gene ID: 5957
Gene Symbol: RCVRN
Immunogen: Synthesized peptide derived from human Recoverin
Immunogen Region: 80-160 aa, Internal
Post Translational Modifications The N-terminal glycine is linked to one of four different types of acyl groups. The most abundant is myristoleate (14:1), but 14:0, 14:2, and 12:0 acyl residues are also present. The Ca(2+) induced exposure of the myristoyl group, known as the calcium-myristoyl switch, promotes RCVRN binding to the photoreceptor cell membranes only when intracellular Ca(2+) concentration is high. Oxidation on Cys-39 occurs in response to prolonged intense illumination and results in the formation of disulfide homodimers, and to a lesser extent disulfide-linked heterodimers.
Function Acts as a calcium sensor and regulates phototransduction of cone and rod photoreceptor cells. Modulates light sensitivity of cone photoreceptor in dark and dim conditions. In response to high Ca(2+) levels induced by low light levels, prolongs RHO/rhodopsin activation in rod photoreceptor cells by binding to and inhibiting GRK1-mediated phosphorylation of RHO/rhodopsin. Plays a role in scotopic vision/enhances vision in dim light by enhancing signal transfer between rod photoreceptors and rod bipolar cells. Improves rod photoreceptor sensitivity in dim light and mediates response of rod photoreceptors to facilitate detection of change and motion in bright light.
Protein Name Recoverin
Cancer-Associated Retinopathy Protein
Protein Car
Database Links Reactome: R-HSA-2514859
Cellular Localisation Photoreceptor Inner Segment
Cell Projection
Cilium
Photoreceptor Outer Segment
Photoreceptor Outer Segment Membrane
Lipid-Anchor
Cytoplasmic Side
Perikaryon
Primarily Expressed In The Inner Segments Of Light-Adapted Rod Photoreceptors
Approximately 10% Of Which Translocates From Photoreceptor Outer Segments Upon Light Stimulation
Targeting Of Myristoylated Protein To Rod Photoreceptor Outer Segments Is Calcium Dependent
Alternative Antibody Names Anti-Recoverin antibody
Anti-Cancer-Associated Retinopathy Protein antibody
Anti-Protein Car antibody
Anti-RCVRN antibody
Anti-RCV1 antibody

Information sourced from Uniprot.org

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