• WB on rat tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody dilution 1: 2000 incubated at 4C overnight.
  • IHC-P on paraffin sections of rat spinal cord.The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
  • IHC-P on paraffin sections of rat spinal cord.The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
  • IHC-P on paraffin sections of rat spinal cord.The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
  • IHC-P on paraffin sections of rat spinal cord.The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
  • IHC-P on paraffin sections of rat olfactory.The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
  • IHC-P on paraffin sections of rat DRG.The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
  • IHC-P on paraffin sections of rat DRG.The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
  • IHC-P on paraffin sections of rat cerebellum.The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
  • IHC-P on paraffin sections of rat brain.The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

Anti-RBFOX3 antibody (STJ13100201)

SKU:
STJ13100201

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Host: NZ White Rabbit
Applications: IHC, WB
Reactivity: Mouse, Rat, Human
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: NZ White Rabbit polyclonal antibody Anti-NeuN is suitable for use in Immunohistochemistry and Western Blot.
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Shipped as lyophilised. Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Purification: Whole serum
Dilution Range: A dilution of 1:1000 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Storage Instruction: Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Gene Symbol: RBFOX3
Gene ID: 146713
Uniprot ID: RFOX3_HUMAN
Immunogen: A synthetic peptide from human NeuN conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse and rat.
Protein Name Rna Binding Protein Fox-1 Homolog 3
Fox-1 Homolog C
Neuronal Nuclei Antigen
Neun Antigen
Cellular Localisation Nucleus
Cytoplasm
Largely Restricted To Neuronal Nuclei
However
Significant Cytoplasmic Localization In Neurons From Brains From Hiv-Infected Individuals With Cognitive Impairment
Alternative Antibody Names Anti-Rna Binding Protein Fox-1 Homolog 3 antibody
Anti-Fox-1 Homolog C antibody
Anti-Neuronal Nuclei Antigen antibody
Anti-Neun Antigen antibody
Anti-RBFOX3 antibody

Information sourced from Uniprot.org

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