• Western blot analysis of extracts of various cell lines, using Ran Rabbit mAb (STJ11102060) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.

Anti-Ran-antibody [ARC0986] (STJ11102060)

SKU:
STJ11102060

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Host: Rabbit
Applications: WB
Reactivity: Human, Mouse, Rat
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit monoclonal antibody anti-Ran is suitable for use in Western Blot.
Clonality: Monoclonal
Clone ID: ARC0986
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Gene Symbol: RAN
Gene ID: 5901
Uniprot ID: RAN_HUMAN
Immunogen: A synthesized peptide derived from human Ran
Tissue Specificity Expressed in a variety of tissues.
Post Translational Modifications Acetylation by KAT5 at Lys-134 is increased during mitosis, impairs RANGRF binding and enhances RCC1 binding. Acetylation at Lys-37 enhances the association with nuclear export components. Deacetylation of Lys-37 by SIRT7 regulates the nuclear export of NF-kappa-B subunit RELA/p65.
Function GTPase involved in nucleocytoplasmic transport, participating both to the import and the export from the nucleus of proteins and RNAs. Switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis. Nuclear import receptors such as importin beta bind their substrates only in the absence of GTP-bound RAN and release them upon direct interaction with GTP-bound RAN, while export receptors behave in the opposite way. Thereby, RAN controls cargo loading and release by transport receptors in the proper compartment and ensures the directionality of the transport. Interaction with RANBP1 induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins. RAN (GTP-bound form) triggers microtubule assembly at mitotic chromosomes and is required for normal mitotic spindle assembly and chromosome segregation. Required for normal progress through mitosis. The complex with BIRC5/survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2. Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.
Protein Name Gtp-Binding Nuclear Protein Ran
Androgen Receptor-Associated Protein 24
Gtpase Ran
Ras-Like Protein Tc4
Ras-Related Nuclear Protein
Database Links Reactome: R-HSA-165054
Reactome: R-HSA-1655829
Reactome: R-HSA-168333
Reactome: R-HSA-180746
Reactome: R-HSA-203927
Reactome: R-HSA-5578749
Reactome: R-HSA-6784531
Reactome: R-HSA-9615933
Cellular Localisation Nucleus
Nucleus Envelope
Cytoplasm
Cytosol
Melanosome
Predominantly Nuclear During Interphase
Becomes Dispersed Throughout The Cytoplasm During Mitosis
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-Gtp-Binding Nuclear Protein Ran antibody
Anti-Androgen Receptor-Associated Protein 24 antibody
Anti-Gtpase Ran antibody
Anti-Ras-Like Protein Tc4 antibody
Anti-Ras-Related Nuclear Protein antibody
Anti-RAN antibody
Anti-ARA24 antibody
Anti-OK antibody
Anti-SW-cl.81 antibody

Information sourced from Uniprot.org

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