Anti-RAB2A antibody (C-Term) (STJ98545)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
ImmunogenPurified recombinant human Rab 2A (C-terminus) protein fragments expressed in E.coli.
General Information
| Short Description | Mouse monoclonal anti-Ras-related protein Rab-2A (C-Term) for use in WB and IF in Human, Mouse, Rat, Cow, Chicken, Dog, Pig and Zebrafish samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF |
| Host | Mouse |
| Reactivity | Human/Mouse/Rat/Cow/Chicken/Dog/Pig/Zebrafish |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity purification |
| Dilution Range | WB 1:1000-1:2000IF 1:100-1:500 |
| Formulation | Liquid in 0.1M Tris-Glycine (pH7.4, 150 mM NaCl) , 0.2% Sodium Azide and 50% Glycerol. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RAB2A |
| Gene ID | 5862 |
| Uniprot ID | RAB2A_HUMAN |
| Immunogen | Purified recombinant human Rab 2A (C-terminus) protein fragments expressed in E.coli. |
| Immunogen Region | C-Term |
| Specificity | Rab 2A Monoclonal Antibody detects endogenous levels of Rab 2A protein. |
Additional Info
| Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between active GTP-bound and inactive GDP-bound states. In their active state, drive transport of vesicular carriers from donor organelles to acceptor organelles to regulate the membrane traffic that maintains organelle identity and morphology. RAB2A regulates autophagy by promoting autophagosome-lysosome fusion via recruitment of the HOPS endosomal tethering complex.this process involves autophagosomal RAB2A and lysosomal RAB39A recruitment of HOPS subcomplexes VPS39-VPS11 and VPS41-VPS16-VPS18-VPS33A, respectively, which assemble into a functional complex to mediate membrane tethering and SNAREs-driven membrane fusion. Required for protein transport from the endoplasmic reticulum to the Golgi complex. Regulates the compacted morphology of the Golgi. Together with RAB2B, redundantly required for efficient autophagic flux. |
| Protein Name | Ras-Related Protein Rab-2a |
| Database Links | Reactome: R-HSA-162658Reactome: R-HSA-8873719 |
| Cellular Localisation | Endoplasmic Reticulum-Golgi Intermediate Compartment MembraneLipid-AnchorCytoplasmic SideMelanosomeEndoplasmic Reticulum MembraneGolgi Apparatus MembraneCytoplasmic VesicleSecretory VesicleAcrosomeAutophagosome MembraneLocalized In The Golgi Apparatus In The Round Spermatids And In The Acrosome In The Elongating SpermatidIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv |
| Alternative Antibody Names | Anti-Ras-Related Protein Rab-2a antibodyAnti-RAB2A antibodyAnti-RAB2 antibody |
Information sourced from Uniprot.org