• Immunofluorescence analysis of MCF-7 cells using PTRH2 antibody (STJ28549). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using PTRH2 antibody (STJ28549) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using PTRH2 antibody (STJ28549) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using PTRH2 antibody (STJ28549) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PTRH2 antibody (40-179) (STJ28549)

SKU:
STJ28549

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PTRH2 (40-179) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:10-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PTRH2
Gene ID: 51651
Uniprot ID: PTH2_HUMAN
Immunogen Region: 40-179
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 40-179 of human PTRH2 (NP_057161.1).
Immunogen Sequence: LPKSKTSKTHTDTESEASIL GDSGEYKMILVVRNDLKMGK GKVAAQCSHAAVSAYKQIQR RNPEMLKQWEYCGQPKVVVK APDEETLIALLAHAKMLGLT VSLIQDAGRTQIAPGSQTVL GIGPGPADLIDKVTGHLKLY
Post Translational Modifications Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.
Function The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis. Promotes caspase-independent apoptosis by regulating the function of two transcriptional regulators, AES and TLE1.
Protein Name Peptidyl-Trna Hydrolase 2 - Mitochondrial
Pth 2
Bcl-2 Inhibitor Of Transcription 1
Database Links Reactome: R-HSA-5689880
Cellular Localisation Mitochondrion Outer Membrane
Single-Pass Membrane Protein
Alternative Antibody Names Anti-Peptidyl-Trna Hydrolase 2 - Mitochondrial antibody
Anti-Pth 2 antibody
Anti-Bcl-2 Inhibitor Of Transcription 1 antibody
Anti-PTRH2 antibody
Anti-BIT1 antibody
Anti-PTH2 antibody
Anti-CGI-147 antibody

Information sourced from Uniprot.org

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