• Western blot analysis of HuvEc cells using PTEN Polyclonal Antibody
  • Western blot analysis of PTEN (Ab-380) Antibody. The lane on the right is blocked with the PTEN (Ab-380) peptide.
  • Western blot analysis of various cells using PTEN Polyclonal Antibody

Anti-PTEN antibody (STJ95257)


Current Stock:
Host: Rabbit
Applications: WB, ELISA
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal anti-PTEN antibody is suitable for use in Western Blot and ELISA.
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG
Dilution Range: WB 1:500-1:2000
ELISA 1:20000
Specificity: PTEN polyclonal antibody detects endogenous levels of PTEN protein.
Gene ID: 5728
Gene Symbol: PTEN
Immunogen: Synthesized peptide derived from human PTEN around the non-phosphorylation site of S380.
Immunogen Region: 320-400 aa
Post Translational Modifications Constitutively phosphorylated by CK2 under normal conditions. Phosphorylated in vitro by MAST1, MAST2, MAST3 and STK11. Phosphorylation results in an inhibited activity towards PIP3. Phosphorylation can both inhibit or promote PDZ-binding. Phosphorylation at Tyr-336 by FRK/PTK5 protects this protein from ubiquitin-mediated degradation probably by inhibiting its binding to NEDD4. Phosphorylation by ROCK1 is essential for its stability and activity. Phosphorylation by PLK3 promotes its stability and prevents its degradation by the proteasome. Monoubiquitinated.monoubiquitination is increased in presence of retinoic acid. Deubiquitinated by USP7.leading to its nuclear exclusion. Monoubiquitination of one of either Lys-13 and Lys-289 amino acid is sufficient to modulate PTEN compartmentalization. Ubiquitinated by XIAP/BIRC4.
Function Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4. The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form induces less tumor suppressive ability. In motile cells, suppresses the formation of lateral pseudopods and thereby promotes cell polarization and directed movement. Isoform alpha: Functional kinase, like isoform 1 it antagonizes the PI3K-AKT/PKB signaling pathway. Plays a role in mitochondrial energetic metabolism by promoting COX activity and ATP production, via collaboration with isoform 1 in increasing protein levels of PINK1.
Protein Name Phosphatidylinositol 3 -4 -5-Trisphosphate 3-Phosphatase And Dual-Specificity Protein Phosphatase Pten
Mutated In Multiple Advanced Cancers 1
Phosphatase And Tensin Homolog
Database Links Reactome: R-HSA-1660499
Reactome: R-HSA-1855204
Reactome: R-HSA-199418
Reactome: R-HSA-202424
Reactome: R-HSA-5628897
Reactome: R-HSA-5674404
Reactome: R-HSA-5689880
Reactome: R-HSA-5689896
Reactome: R-HSA-8943723
Reactome: R-HSA-8948747
Reactome: R-HSA-8948751
Reactome: R-HSA-8986944
Cellular Localisation Cytoplasm
Pml Body
Monoubiquitinated Form Is Nuclear
Nonubiquitinated Form Is Cytoplasmic
Colocalized With Pml And Usp7 In Pml Nuclear Bodies
Xiap/Birc4 Promotes Its Nuclear Localization
Isoform Alpha: Secreted
May Be Secreted Via A Classical Signal Peptide And Reenter Into Cells With The Help Of A Poly-Arg Motif
Alternative Antibody Names Anti-Phosphatidylinositol 3 -4 -5-Trisphosphate 3-Phosphatase And Dual-Specificity Protein Phosphatase Pten antibody
Anti-Mutated In Multiple Advanced Cancers 1 antibody
Anti-Phosphatase And Tensin Homolog antibody
Anti-PTEN antibody
Anti-MMAC1 antibody
Anti-TEP1 antibody

Information sourced from Uniprot.org

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