• Immunofluorescence analysis of U-251MG cells using PRPF8 Rabbit polyclonal antibody (STJ27847) at dilution of 1:100 (20x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of PRPF8 in paraffin-embedded rat pancreas using PRPF8 Rabbit polyclonal antibody (STJ27847) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of PRPF8 in paraffin-embedded mouse brain using PRPF8 Rabbit polyclonal antibody (STJ27847) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of PRPF8 in paraffin-embedded human colon carcinoma using PRPF8 Rabbit polyclonal antibody (STJ27847) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using PRPF8 Rabbit polyclonal antibody (STJ27847) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PRPF8 antibody (1-100) (STJ27847)

SKU:
STJ27847

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PRPF8 (1-100) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRPF8
Gene ID: 10594
Uniprot ID: PRP8_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PRPF8 (NP_006436.3).
Immunogen Sequence: MAGVFPYRGPGNPVPGPLAP LPDYMSEEKLQEKARKWQQL QAKRYAEKRKFGFVDAQKED MPPEHVRKIIRDHGDMTNRK FRHDKRVYLGALKYMPHAVL
Tissue Specificity Widely expressed.
Function Plays a role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes, both of the predominant U2-type spliceosome and the minor U12-type spliceosome. Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex, a building block of the spliceosome. Functions as scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site.
Protein Name Pre-Mrna-Processing-Splicing Factor 8
220 Kda U5 Snrnp-Specific Protein
Prp8 Homolog
Splicing Factor Prp8
P220
Database Links Reactome: R-HSA-72163
Reactome: R-HSA-72165
Cellular Localisation Nucleus
Nucleus Speckle
Alternative Antibody Names Anti-Pre-Mrna-Processing-Splicing Factor 8 antibody
Anti-220 Kda U5 Snrnp-Specific Protein antibody
Anti-Prp8 Homolog antibody
Anti-Splicing Factor Prp8 antibody
Anti-P220 antibody
Anti-PRPF8 antibody
Anti-PRPC8 antibody

Information sourced from Uniprot.org

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