• Western blot analysis of extracts of various cell lines, using PRDM5 antibody (STJ29499) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry of paraffin-embedded rat spleen using PRDM5 antibody (STJ29499) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse liver using PRDM5 antibody (STJ29499) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse spleen using PRDM5 antibody (STJ29499) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of A549 cells using PRDM5 antibody (STJ29499). Blue: DAPI for nuclear staining.

Anti-PRDM5 antibody (1-100) (STJ29499)

SKU:
STJ29499

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PRDM5 (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRDM5
Gene ID: 11107
Uniprot ID: PRDM5_HUMAN
Immunogen Region: 1-100
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human PRDM5 (NP_061169.2).
Immunogen Sequence: MLGMYVPDRFSLKSSRVQDG MGLYTARRVRKGEKFGPFAG EKRMPEDLDENMDYRLMWEV RGSKGEVLYILDATNPRHSN WLRFVHEAPSQEQKNLAAIQ
Tissue Specificity Widely expressed with highest levels in colon and ovary. Tends to be silenced in breast, colorectal, gastric and liver cancer tissues.
Function Sequence-specific DNA-binding transcription factor. Represses transcription at least in part by recruitment of the histone methyltransferase EHMT2/G9A and histone deacetylases such as HDAC1. Regulates hematopoiesis-associated protein-coding and microRNA (miRNA) genes. May regulate the expression of proteins involved in extracellular matrix development and maintenance, including fibrillar collagens, such as COL4A1 and COL11A1, connective tissue components, such as HAPLN1, and molecules regulating cell migration and adhesion, including EDIL3 and TGFB2. May cause G2/M arrest and apoptosis in cancer cells.
Protein Name Pr Domain Zinc Finger Protein 5
Pr Domain-Containing Protein 5
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Pr Domain Zinc Finger Protein 5 antibody
Anti-Pr Domain-Containing Protein 5 antibody
Anti-PRDM5 antibody
Anti-PFM2 antibody

Information sourced from Uniprot.org

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