• Immunohistochemical analysis of paraffin-embedded Mouse Heart Tissue using PPAR-Gamma Polyclonal Antibody.
  • Western blot analysis of lysates from 1) Hela, 2) 3T3, 3) PC12 cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta monoclonal antibody (5B7) (cat: (STJ96930) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.
  • Western blot analysis of 1) Hela, 2) 3T3, 3) PC12 using PPAR-Gamma Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-PPAR-Gamma antibody (STJ97460)

SKU:
STJ97460

Current Stock:
Host: Rabbit
Applications: WB, IHC-P
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal anti-PPAR-Gamma antibody is suitable for use in Western Blot and Immunohistochemistry.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS, pH 7.4, containing 0.5% BSA, 0.02% sodium azide and 50% Glycerol.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/mL
Isotype: IgG
Dilution Range: WB 1:500-1000
IHC 1:200-500
Specificity: The antibody detects endogenous PPAR-gamma protein.
Uniprot ID: PPARG_HUMAN
Gene ID: 5468
Gene Symbol: PPARG
Immunogen: Recombinant Protein
Post Translational Modifications O-GlcNAcylation at Thr-84 reduces transcriptional activity in adipocytes. Phosphorylated in basal conditions and dephosphorylated when treated with the ligand. May be dephosphorylated by PPP5C. The phosphorylated form may be inactive and dephosphorylation at Ser-112 induces adipogenic activity.
Function Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of ARNTL/BMAL1 in the blood vessels. (Microbial infection) Upon treatment with M.tuberculosis or its lipoprotein LpqH, phosphorylation of MAPK p38 and IL-6 production are modulated, probably via this protein.
Protein Name Peroxisome Proliferator-Activated Receptor Gamma
Ppar-Gamma
Nuclear Receptor Subfamily 1 Group C Member 3
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-381340
Reactome: R-HSA-383280
Reactome: R-HSA-4090294
Reactome: R-HSA-8943724
Reactome: R-HSA-9022707
Cellular Localisation Nucleus
Cytoplasm
Redistributed From The Nucleus To The Cytosol Through A Map2k1/Mek1-Dependent Manner
Noct Enhances Its Nuclear Translocation
Alternative Antibody Names Anti-Peroxisome Proliferator-Activated Receptor Gamma antibody
Anti-Ppar-Gamma antibody
Anti-Nuclear Receptor Subfamily 1 Group C Member 3 antibody
Anti-PPARG antibody
Anti-NR1C3 antibody

Information sourced from Uniprot.org

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