• Western blot analysis of extracts of various cell lines, using PPARγ antibody (STJ11101758) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 3min.

Anti-PPAR gamma-antibody [ARC0155] (STJ11101758)

SKU:
STJ11101758

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Host: Rabbit
Applications: WB
Reactivity: Rat
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit monoclonal antibody anti-PPAR gamma is suitable for use in Western Blot.
Clonality: Monoclonal
Clone ID: ARC0155
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Gene Symbol: PPARG
Gene ID: 5468
Uniprot ID: PPARG_HUMAN
Immunogen: A synthesized peptide derived from human PPAR gamma.
Tissue Specificity Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary.
Post Translational Modifications O-GlcNAcylation at Thr-84 reduces transcriptional activity in adipocytes. Phosphorylated in basal conditions and dephosphorylated when treated with the ligand. May be dephosphorylated by PPP5C. The phosphorylated form may be inactive and dephosphorylation at Ser-112 induces adipogenic activity.
Function Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of ARNTL/BMAL1 in the blood vessels. (Microbial infection) Upon treatment with M.tuberculosis or its lipoprotein LpqH, phosphorylation of MAPK p38 and IL-6 production are modulated, probably via this protein.
Protein Name Peroxisome Proliferator-Activated Receptor Gamma
Ppar-Gamma
Nuclear Receptor Subfamily 1 Group C Member 3
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-381340
Reactome: R-HSA-383280
Reactome: R-HSA-4090294
Reactome: R-HSA-8943724
Reactome: R-HSA-9022707
Cellular Localisation Nucleus
Cytoplasm
Redistributed From The Nucleus To The Cytosol Through A Map2k1/Mek1-Dependent Manner
Noct Enhances Its Nuclear Translocation
Alternative Antibody Names Anti-Peroxisome Proliferator-Activated Receptor Gamma antibody
Anti-Ppar-Gamma antibody
Anti-Nuclear Receptor Subfamily 1 Group C Member 3 antibody
Anti-PPARG antibody
Anti-NR1C3 antibody

Information sourced from Uniprot.org

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