• STJ70468 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/membrane and nuclear staining. The nuc
  • STJ70468 staining (0.01µg/ml) of Human Parathyroid lysate (A) and negative control HepG2 (B) cell lysate (35µg protein in RIPA buffer). . Detected by chemiluminescence.
  • STJ70468 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol
  • STJ70468 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/membrane and nuclear staining. The nuc
  • STJ70468 staining (0.01µg/ml) of Human Parathyroid lysate (A) and negative control HepG2 (B) cell lysate (35µg protein in RIPA buffer). . Detected by chemiluminescence.
  • STJ70468 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol

Anti-Pleckstrin Antibody (STJ70468)

SKU:
STJ70468

Current Stock:
Host: Goat
Applications: Pep-ELISA, WB, IF, FC, IHC
Reactivity: Human
Short Description: Goat polyclonal anti-Pleckstrin antibody is suitable for use in ELISA, Western Blot, Immunofluorescence, Flow Cytometry and Immunohistochemistry.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at-20°C. Minimize freezing and thawing.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage Instruction: Store at-20°C and avoid freeze-thaw cycles.
Concentration: 0.5mg/mL
Isotype: IgG
Dilution Range: WB-0.01-0.3µg/ml
IHC-3.75µg/ml
IF-Strong expression of the protein seen in the cytoplasm/membranes and nuclei of HeLa cells. 10µg/ml
FC-Flow cytometric analysis of HeLa cells. 10ug/ml
ELISA-antibody detection limit dilution 1:16000.
Uniprot ID: PLEK_HUMAN
Gene ID: 5341
Gene Symbol: PLEK
Immunogen Region: C Terminus 350 aa
Immunogen Sequence: EWIKAIQMASRTGK
Accession Number: NP_002655.2
Protein Name Pleckstrin
Platelet 47 Kda Protein
P47
Database Links Reactome: R-HSA-114608
Alternative Antibody Names Anti-Pleckstrin antibody
Anti-Platelet 47 Kda Protein antibody
Anti-P47 antibody
Anti-PLEK antibody
Anti-P47 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance