• Western blot analysis of lysates from 293 cells, treated with EGF 200ng/ml 30', using PLCG1 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using PLCG1 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using PLC Gamma 1 Polyclonal Antibody diluted at 1:2000

Anti-PLCG1-Gamma antibody (720-800 aa) (STJ95158)

SKU:
STJ95158

Current Stock:
Host: Rabbit
Applications: WB, IHC-P, ELISA
Reactivity: Human, Mouse, Rat
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit polyclonal antibody Anti-1-phosphatidylinositol 4, 5-bisphosphate phosphodiesterase gamma-1 (720-800 aa) is suitable for use in Western Blot, Immunohistochemistry and ELISA.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Concentration: 1 mg/ml
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Gene Symbol: PLCG1
Gene ID: 5335
Uniprot ID: PLCG1_HUMAN
Immunogen Region: 720-800 aa
Specificity: PLC gamma 1 polyclonal antibody detects endogenous levels of PLC gamma 1 protein.
Immunogen: Synthesized peptide derived from human PLC gamma1 around the non-phosphorylation site of Y783.
Function Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, EGFR, FGFR1, FGFR2, FGFR3 and FGFR4. Plays a role in actin reorganization and cell migration.
Protein Name 1-Phosphatidylinositol 4 -5-Bisphosphate Phosphodiesterase Gamma-1
Plc-148
Phosphoinositide Phospholipase C-Gamma-1
Phospholipase C-Ii
Plc-Ii
Phospholipase C-Gamma-1
Plc-Gamma-1
Database Links Reactome: R-HSA-1169408
Reactome: R-HSA-1236382
Reactome: R-HSA-1251932
Reactome: R-HSA-1489509
Reactome: R-HSA-167021
Reactome: R-HSA-1855204
Reactome: R-HSA-186763
Reactome: R-HSA-202433
Reactome: R-HSA-2029485
Reactome: R-HSA-210990
Reactome: R-HSA-212718
Reactome: R-HSA-2424491
Reactome: R-HSA-2871796
Reactome: R-HSA-2871809
Reactome: R-HSA-418890
Reactome: R-HSA-5218921
Reactome: R-HSA-5637810
Reactome: R-HSA-5654219
Reactome: R-HSA-5654221
Reactome: R-HSA-5654227
Reactome: R-HSA-5654228
Reactome: R-HSA-5655253
Reactome: R-HSA-5655291
Reactome: R-HSA-5655302
Reactome: R-HSA-8853338
Reactome: R-HSA-8853659
Reactome: R-HSA-9026527
Reactome: R-HSA-9027277
Reactome: R-HSA-9034793
Reactome: R-HSA-9664323
Reactome: R-HSA-9664565
Reactome: R-HSA-9665348
Reactome: R-HSA-9665686
Cellular Localisation Cell Projection
Lamellipodium
Ruffle
Rapidly Redistributed To Ruffles And Lamellipodia Structures In Response To Epidermal Growth Factor (Egf) Treatment
Alternative Antibody Names Anti-1-Phosphatidylinositol 4 -5-Bisphosphate Phosphodiesterase Gamma-1 antibody
Anti-Plc-148 antibody
Anti-Phosphoinositide Phospholipase C-Gamma-1 antibody
Anti-Phospholipase C-Ii antibody
Anti-Plc-Ii antibody
Anti-Phospholipase C-Gamma-1 antibody
Anti-Plc-Gamma-1 antibody
Anti-PLCG1 antibody
Anti-PLC1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance