• Western blot analysis of TF-1 cells, using Phospho-STAT5A-Y694 rabbit monoclonal antibody (STJ11102567) at 1:1000 dilution. TF-1 cells were treated by GM-CSF (25 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using Phospho-STAT5A-Y694 antibody (STJ11102567) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using Phospho-STAT5A-Y694 rabbit monoclonal antibody (STJ11102567) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using Phospho-STAT5A-Y694 rabbit monoclonal antibody (STJ11102567) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human kidney using Phospho-STAT5A-Y694 rabbit monoclonal antibody (STJ11102567) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver using Phospho-STAT5A-Y694 rabbit monoclonal antibody (STJ11102567) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human placenta using Phospho-STAT5A-Y694 rabbit monoclonal antibody (STJ11102567) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human spleen using Phospho-STAT5A-Y694 rabbit monoclonal antibody (STJ11102567) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using Phospho-STAT5A-Y694 rabbit monoclonal antibody (STJ11102567) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human tonsil using Phospho-STAT5A-Y694 rabbit monoclonal antibody (STJ11102567) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse intestin using Phospho-STAT5A-Y694 rabbit monoclonal antibody (STJ11102567) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse liver using Phospho-STAT5A-Y694 rabbit monoclonal antibody (STJ11102567) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using Phospho-STAT5A-Y694 rabbit monoclonal antibody (STJ11102567) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-STAT5A-Y694 antibody [S7MR] (STJ11102567)

SKU:
STJ11102567

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Phospho-STAT5A-Y694 is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STAT5A
Gene ID: 6776
Uniprot ID: STA5A_HUMAN
Immunogen: A synthetic phosphorylated peptide around Y694 of human Stat5 (P42229).
Immunogen Sequence: DGYVK
Post Translational Modifications Tyrosine phosphorylated in response to KITLG/SCF, IL2, IL3, IL7, IL15, CSF2/GMCSF, GH1, PRL, EPO and THPO. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. Tyrosine phosphorylated in response to signaling via activated FLT3.wild-type FLT3 results in much weaker phosphorylation than constitutively activated mutant FLT3. Alternatively, can be phosphorylated by JAK2 at Tyr-694. ISGylated.
Function Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.
Protein Name Signal Transducer And Activator Of Transcription 5a
Database Links Reactome: R-HSA-1170546
Reactome: R-HSA-1251985
Reactome: R-HSA-1266695
Reactome: R-HSA-1433557
Reactome: R-HSA-1839117
Reactome: R-HSA-186763
Reactome: R-HSA-2586552
Reactome: R-HSA-512988
Reactome: R-HSA-8854691
Reactome: R-HSA-8983432
Reactome: R-HSA-8985947
Reactome: R-HSA-9020558
Reactome: R-HSA-9020958
Reactome: R-HSA-9027283
Reactome: R-HSA-9645135
Reactome: R-HSA-9670439
Reactome: R-HSA-9674555
Reactome: R-HSA-9702518
Reactome: R-HSA-9703465
Reactome: R-HSA-9705462
Reactome: R-HSA-9725371
Reactome: R-HSA-982772
Cellular Localisation Cytoplasm
Nucleus
Translocated Into The Nucleus In Response To Phosphorylation
Alternative Antibody Names Anti-Signal Transducer And Activator Of Transcription 5a antibody
Anti-STAT5A antibody
Anti-STAT5 antibody

Information sourced from Uniprot.org

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