• Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using Muscle Actin Mouse mAb diluted at 1:200.

Anti-Phospho-Stat3 (Y705) antibody [1E9] (STJ97747)


Current Stock:
Host: Mouse
Applications: IHC-P
Reactivity: Human
Short Description: Mouse monoclonal anti-Phospho-Stat3 (Y705) antibody is suitable for use in Immunohistochemistry.
Clonality: Monoclonal
Clone ID: 1E9
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using a specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG1
Dilution Range: IHC 1:100-200
Specificity: Phospho-Stat3 (Y705) protein detects endogenous levels of STAT3
Gene ID: 6774
Gene Symbol: STAT3
Immunogen: synthetic peptide derived from Phospho-Stat3 (Y705)
Immunogen Region: 640-720 aa
Post Translational Modifications Tyrosine phosphorylated upon stimulation with EGF. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4. Activated through tyrosine phosphorylation by BMX. Tyrosine phosphorylated in response to IL6, IL11, LIF, CNTF, KITLG/SCF, CSF1, EGF, PDGF, IFN-alpha, LEP and OSM. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus. Upon LPS challenge, phosphorylated within the nucleus by IRAK1. Upon erythropoietin treatment, phosphorylated on Ser-727 by RPS6KA5. Phosphorylation at Tyr-705 by PTK6 or FER leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates IL6/interleukin-6 signaling. Acetylated on lysine residues by CREBBP. Deacetylation by LOXL3 leads to disrupt STAT3 dimerization and inhibit STAT3 transcription activity. Oxidation of lysine residues to allysine on STAT3 preferentially takes place on lysine residues that are acetylated. Some lysine residues are oxidized to allysine by LOXL3, leading to disrupt STAT3 dimerization and inhibit STAT3 transcription activity. Oxidation of lysine residues to allysine on STAT3 preferentially takes place on lysine residues that are acetylated. (Microbial infection) Phosphorylated on Tyr-705 in the presence of S.typhimurium SarA.
Function Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Upon activation of IL6ST/gp130 signaling by interleukin-6 (IL6), binds to the IL6-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Acts as a regulator of inflammatory response by regulating differentiation of naive CD4(+) T-cells into T-helper Th17 or regulatory T-cells (Treg): deacetylation and oxidation of lysine residues by LOXL3, leads to disrupt STAT3 dimerization and inhibit its transcription activity. Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1. Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation. May play an apoptotic role by transctivating BIRC5 expression under LEP activation. Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity. Plays a crucial role in basal beta cell functions, such as regulation of insulin secretion.
Protein Name Signal Transducer And Activator Of Transcription 3
Acute-Phase Response Factor
Database Links Reactome: R-HSA-1059683
Reactome: R-HSA-111453
Reactome: R-HSA-1266695
Reactome: R-HSA-1433557
Reactome: R-HSA-1839117
Reactome: R-HSA-186763
Reactome: R-HSA-198745
Reactome: R-HSA-2559582
Reactome: R-HSA-2586552
Reactome: R-HSA-2892247
Reactome: R-HSA-390471
Reactome: R-HSA-452723
Reactome: R-HSA-6783783
Reactome: R-HSA-6785807
Reactome: R-HSA-8849474
Reactome: R-HSA-8854691
Reactome: R-HSA-8875791
Reactome: R-HSA-8983432
Reactome: R-HSA-8984722
Reactome: R-HSA-8985947
Reactome: R-HSA-9008059
Reactome: R-HSA-9020933
Reactome: R-HSA-9020956
Reactome: R-HSA-9020958
Reactome: R-HSA-9616222
Reactome: R-HSA-9670439
Reactome: R-HSA-9673767
Reactome: R-HSA-9673770
Reactome: R-HSA-982772
Cellular Localisation Cytoplasm
Shuttles Between The Nucleus And The Cytoplasm
Translocated Into The Nucleus Upon Tyrosine Phosphorylation And Dimerization
In Response To Signaling By Activated Fgfr1
Fgfr3 Or Fgfr4
Constitutive Nuclear Presence Is Independent Of Tyrosine Phosphorylation
Predominantly Present In The Cytoplasm Without Stimuli
Upon Leukemia Inhibitory Factor (Lif) Stimulation
Accumulates In The Nucleus
The Complex Composed Of Bart And Arl2 Plays An Important Role In The Nuclear Translocation And Retention Of Stat3
Identified In A Complex With Lyn And Pag1
Alternative Antibody Names Anti-Signal Transducer And Activator Of Transcription 3 antibody
Anti-Acute-Phase Response Factor antibody
Anti-STAT3 antibody
Anti-APRF antibody

Information sourced from Uniprot.org

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