• Western blot analysis of lysates from COS7 cells, using STAT1 (Phospho-Tyr701) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of lysates from 1) MOUSE-BRAIN, 2) SY-SY5X, 3) COS7 cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta monoclonal antibody (5B7) (cat: (STJ96930) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.
  • Western blot analysis of COS7 cells using Phospho-Stat1 (Y701) Polyclonal Antibody diluted at 1：1000
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using STAT1 (Phospho-Tyr701) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue. 1, Stat1 (phospho Tyr701) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Western blot analysis of various cells using Phospho-Stat1 (Y701) Polyclonal Antibody diluted at 1：1000
  • Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, Stat1 (phospho Tyr701) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1, Stat1 (phospho Tyr701) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunofluorescence analysis of mouse-spleen tissue. 1, Stat1 (phospho Tyr701) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of mouse-spleen tissue. 1, Stat1 (phospho Tyr701) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of rat-spleen tissue. 1, Stat1 (phospho Tyr701) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of rat-spleen tissue. 1, Stat1 (phospho Tyr701) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of Hela cell. 1, Stat1 (phospho Tyr701) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). HER2 monoclonal antibody (11H9) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-STAT1-Tyr701 antibody (668-717 aa) (STJ90409)

SKU:
STJ90409

$62.10 - $305.10
Current Stock:
Host: Rabbit
Applications: IF/WB/IHC/IP/ELISA
Reactivity: Human/Mouse/Rat/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Signal transducer and activator of transcription 1-alpha/beta-Tyr701 (668-717 aa) is suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry, Immunoprecipitation and ELISA research applicatio
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IF 1:50-200
WB 1:500-1:2000
IHC 1:100-1:300
IP 2-5 ug mg/lysate
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STAT1
Gene ID: 6772
Uniprot ID: STAT1_HUMAN
Immunogen Region: 668-717 aa
Specificity: Phospho-Stat1 (Y701) Polyclonal Antibody detects endogenous levels of Stat1 protein only when phosphorylated at Y701.
Immunogen: The antiserum was produced against synthesized peptide derived from the human STAT1 around the phosphorylation site of Tyr701 at the amino acid range 668-717
Function Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Involved in food tolerance in small intestine: associates with the Gasdermin-D, p13 cleavage product (13 kDa GSDMD) and promotes transcription of CIITA, inducing type 1 regulatory T (Tr1) cells in upper small intestine.
Protein Name Signal Transducer And Activator Of Transcription 1-Alpha/Beta
Transcription Factor Isgf-3 Components P91/P84
Database Links Reactome: R-HSA-1059683 P42224-1
Reactome: R-HSA-1169408
Reactome: R-HSA-1433557 P42224-1
Reactome: R-HSA-1839117
Reactome: R-HSA-186763
Reactome: R-HSA-2173795 P42224-1
Reactome: R-HSA-6785807
Reactome: R-HSA-877300 P42224-1
Reactome: R-HSA-877312 P42224-1
Reactome: R-HSA-8854691
Reactome: R-HSA-8939902
Reactome: R-HSA-8984722
Reactome: R-HSA-8985947
Reactome: R-HSA-9013508
Reactome: R-HSA-9020956
Reactome: R-HSA-9020958
Reactome: R-HSA-909733
Reactome: R-HSA-912694
Reactome: R-HSA-9670439 P42224-1
Reactome: R-HSA-9673767
Reactome: R-HSA-9673770
Reactome: R-HSA-9674555
Reactome: R-HSA-9680350
Reactome: R-HSA-9705462
Reactome: R-HSA-9705671
Reactome: R-HSA-982772
Cellular Localisation Cytoplasm
Nucleus
Translocated Into The Nucleus Upon Tyrosine Phosphorylation And Dimerization
In Response To Ifn-Gamma And Signaling By Activated Fgfr1
Fgfr2
Fgfr3 Or Fgfr4
Monomethylation At Lys-525 Is Required For Phosphorylation At Tyr-701 And Translocation Into The Nucleus
Translocates Into The Nucleus In Response To Interferon-Beta Stimulation
Alternative Antibody Names Anti-Signal Transducer And Activator Of Transcription 1-Alpha/Beta antibody
Anti-Transcription Factor Isgf-3 Components P91/P84 antibody
Anti-STAT1 antibody

Information sourced from Uniprot.org

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