• Western blot analysis of extracts of 293T cells, using Phospho-PKA RII Alpha (PRKAR2A) -S99 rabbit monoclonal antibody (STJ11102613) at 1:1000 dilution. 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of C6 cells, using Phospho-PKA RII Alpha (PRKAR2A) -S99 Rabbit monoclonal antibody (STJ11102613) at 1:1000 dilution. C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3min.
  • Western blot analysis of extracts of C6 cells, using Phospho-PKA RII Alpha (PRKAR2A) -S99 rabbit monoclonal antibody (STJ11102613) at 1:1000 dilution. C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3min.
  • Western blot analysis of extracts of 293T cells, using Phospho-PKA RII Alpha (PRKAR2A) -S99 Rabbit monoclonal antibody (STJ11102613) at 1:1000 dilution. 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-PRKAR2A-S99 antibody [S3MR] (STJ11102613)

SKU:
STJ11102613

£212.50 - £501.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Phospho-PKA R2-S99 is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRKAR2A
Gene ID: 5576
Uniprot ID: KAP2_HUMAN
Immunogen: A synthetic phosphorylated peptide around S99 of human PRKAR2A/PKR2 (P13861).
Immunogen Sequence: RVSVC
Tissue Specificity Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
Post Translational Modifications Phosphorylated by the activated catalytic chain.
Function Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
Protein Name Camp-Dependent Protein Kinase Type Ii-Alpha Regulatory Subunit
Database Links Reactome: R-HSA-163615
Reactome: R-HSA-164378
Reactome: R-HSA-180024
Reactome: R-HSA-381676
Reactome: R-HSA-432040
Reactome: R-HSA-442720
Reactome: R-HSA-5610787
Reactome: R-HSA-9010642
Reactome: R-HSA-9634597
Reactome: R-HSA-9660821
Reactome: R-HSA-9664323
Reactome: R-HSA-983231
Cellular Localisation Cytoplasm
Cell Membrane
Colocalizes With Pja2 In The Cytoplasm And The Cell Membrane
Alternative Antibody Names Anti-Camp-Dependent Protein Kinase Type Ii-Alpha Regulatory Subunit antibody
Anti-PRKAR2A antibody
Anti-PKR2 antibody
Anti-PRKAR2 antibody

Information sourced from Uniprot.org

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