• Western blot analysis of extracts of various cell lines, using Phospho-Paxillin-Y118 rabbit monoclonal antibody (STJ11102648) at 1:1000 dilution. A-431 cells were treated by pervanadate (1 mM) at 37 °C for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by pervanadate (1 mM) at 37 °C for 30 minutes after serum-starvation overnight. C6 cells were treated by pervanadate (1 mM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of various cell lines, using Phospho-Paxillin-Y118 Rabbit monoclonal antibody (STJ11102648) at 1:1000 dilution. A-431 cells were treated by pervanadate (1 mM) at 37 °C for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by pervanadate (1 mM) at 37 °C for 30 minutes after serum-starvation overnight. C6 cells were treated by pervanadate (1 mM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-PXN-Y118 antibody [S8MR] (STJ11102648)

SKU:
STJ11102648

£212.50 - £501.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Phospho-Paxillin-Y118 is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PXN
Gene ID: 5829
Uniprot ID: PAXI_HUMAN
Immunogen: A synthetic phosphorylated peptide around Y118 of human Paxillin (P49023).
Immunogen Sequence: HVYSF
Post Translational Modifications Phosphorylated by MAPK1/ERK2. Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. Phosphorylation at Ser-244 by CDK5 reduces its interaction with PTK2/FAK1 in matrix-cell focal adhesions (MCFA) during oligodendrocytes (OLs) differentiation. Phosphorylation at Tyr-31 and Tyr-118 by PTK6 promote the activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. Phosphorylation at Ser-250 by SLK is required for PXN redistribution and cell motility.
Function Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Recruits other proteins such as TRIM15 to focal adhesion.
Protein Name Paxillin
Database Links Reactome: R-HSA-180292
Reactome: R-HSA-4420097
Reactome: R-HSA-445355
Reactome: R-HSA-446343
Reactome: R-HSA-446388
Reactome: R-HSA-8849471
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Junction
Focal Adhesion
Cell Cortex
Colocalizes With Integrins At The Cell Periphery
Colocalize With Pxn To Membrane Ruffles And The Leading Edge Of Migrating Cells
Alternative Antibody Names Anti-Paxillin antibody
Anti-PXN antibody

Information sourced from Uniprot.org

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