• Western blot analysis of extracts of Jurkat cells, using Pan Phospho-Tyrosine polyclonal antibody (STJ11101040) at 1:1000 dilution. Jurkat cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of NIH/3T3 cells using Pan Phospho-Tyrosine (STJ11101040) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. NIH/3T3 cells were treated by 20% FBS at 37 °C for 20 minutes after serum-starvation overnight.
  • Immunofluorescence analysis of C6 cells using Pan Phospho-Tyrosine (STJ11101040) at dilution of 1:100. Blue: DAPI for nuclear staining. C6 cells were treated by 20% FBS at 37 °C for 20 minutes after serum-starvation overnight.
  • Immunofluorescence analysis of C6 cells using Pan Phospho-Tyrosine (STJ11101040) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. C6 cells were treated by 20% FBS at 37 °C for 20 minutes after serum-starvation overnight.
  • Immunofluorescence analysis of NIH/3T3 cells using Pan Phospho-Tyrosine (STJ11101040) at dilution of 1:100. Blue: DAPI for nuclear staining. NIH/3T3 cells were treated by 20% FBS at 37 °C for 20 minutes after serum-starvation overnight.
  • Western blot analysis of lysates from Jurkat cells, using Pan Phospho-Tyrosine polyclonal antibody (STJ11101040) at 1:1000 dilution. Jurkat cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug Pan Phospho-Tyrosine antibody (STJ11101040). Western blot was performed from the immunoprecipitate using Pan Phospho-Tyrosine antibody (STJ11101040) at a dilition of 1:1000. Jurkat cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes.

Anti-Pan-Phospho-Tyrosine antibody (STJ11101040)

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STJ11101040
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Background Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Located in fibrillar center and microtubule cytoskeleton.

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