Anti-Phospho-NEK9-Thr210 antibody (176-225 aa) (STJ90724)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human NEK9 around the phosphorylation site of Thr210 at the amino acid range 176-225
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Serine/threonine-protein kinase Nek9-Thr210 (176-225 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NEK9 |
| Gene ID | 91754 |
| Uniprot ID | NEK9_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human NEK9 around the phosphorylation site of Thr210 at the amino acid range 176-225 |
| Immunogen Region | 176-225 aa |
| Specificity | Phospho-Nek9 (T210) Polyclonal Antibody detects endogenous levels of Nek9 protein only when phosphorylated at T210. |
Additional Info
| Post Translational Modifications | Autophosphorylated on serine and threonine residues. When complexed with FACT, exhibits markedly elevated phosphorylation on Thr-210. During mitosis, not phosphorylated on Thr-210. Phosphorylated by CDK1 in vitro. |
| Function | Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression. Phosphorylates NEK6 and NEK7 and stimulates their activity by releasing the autoinhibitory functions of Tyr-108 and Tyr-97 respectively. |
| Protein Name | Serine/Threonine-Protein Kinase Nek9Nercc1 KinaseNever In Mitosis A-Related Kinase 9Nima-Related Protein Kinase 9Nima-Related Kinase 8Nek8 |
| Database Links | Reactome: R-HSA-2980767Reactome: R-HSA-3301854Reactome: R-HSA-9648025 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Nek9 antibodyAnti-Nercc1 Kinase antibodyAnti-Never In Mitosis A-Related Kinase 9 antibodyAnti-Nima-Related Protein Kinase 9 antibodyAnti-Nima-Related Kinase 8 antibodyAnti-Nek8 antibodyAnti-NEK9 antibodyAnti-KIAA1995 antibodyAnti-NEK8 antibodyAnti-NERCC antibody |
Information sourced from Uniprot.org