• Immunohistochemical analysis of paraffin-embedded Human Skin Tissue using Phospho-MLKL S358 Mouse mAb diluted at 1:200.
  • Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using Phospho-MLKL S358 Mouse mAb diluted at 1:200.

Anti-phospho-MLKL (S358) antibody [6B4] (STJ97777)

SKU:
STJ97777

Current Stock:
Host: Mouse
Applications: IHC-P
Reactivity: Human
Short Description: Mouse monoclonal anti-phospho-MLKL (S358) antibody is suitable for use in Immunohistochemistry.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Monoclonal
Clone ID: 6B4
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using a specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG1
Dilution Range: WB 1:500-1:2000
IHC-p 1:50-200
Specificity: phospho-MLKL (S358) protein detects endogenous levels of MLKL
Uniprot ID: MLKL_HUMAN
Gene ID: 197259
Gene Symbol: MLKL
Immunogen: synthetic peptide derived from phospho-MLKL (S358)
Function Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Does not have protein kinase activity. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following activation by ZBP1, MLKL is phosphorylated by RIPK3 in the nucleus, triggering disruption of the nuclear envelope and leakage of cellular DNA into the cytosol.following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol. Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function.
Protein Name Mixed Lineage Kinase Domain-Like Protein
Hmlkl
Database Links Reactome: R-HSA-3295583
Reactome: R-HSA-5213460
Reactome: R-HSA-5675482
Reactome: R-HSA-9686347
Cellular Localisation Cytoplasm
Cell Membrane
Nucleus
Localizes To The Cytoplasm And Translocates To The Plasma Membrane On Necroptosis Induction
Localizes To The Nucleus In Response To Orthomyxoviruses Infection
Alternative Antibody Names Anti-Mixed Lineage Kinase Domain-Like Protein antibody
Anti-Hmlkl antibody
Anti-MLKL antibody

Information sourced from Uniprot.org

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